Investigation of lipolytic activity of the red king crab hepatopancreas homogenate by NMR spectroscopy
Maria Timchenko,
Vladislav Molchanov,
Maxim Molchanov,
Alexander Timchenko,
Evgeny Sogorin
Affiliations
Maria Timchenko
Laboratory of NMR biosystems, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia
Vladislav Molchanov
Medical Physics Department, Dubna State University, Branch Protvino, Protvino, Russia
Maxim Molchanov
Laboratory of NMR biosystems, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia
Alexander Timchenko
Group of Experimental Research and Engineering Oligomeric Structures, Institute of Protein Research, Russian Academy of Sciences, Pushchino, Russia
Evgeny Sogorin
Institute for Biological Instrumentation, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, Pushchino, Russia
The digestive gland of craboids (hepatopancreas) is rich in a huge number of various enzymes (collagenases, nucleases, hyaluronidases, proteases), which are well studied at the moment. However, little is known about crustacean lipases. In this work, using 1H NMR spectroscopy, it was found that the hepatopancreas homogenate of the red king crab Paralithodes camtschaticus demonstrates high lipolytic activity against triacetin in a wide pH range and shows moderate activity against the caprylic/capric triglyceride emulsion. Under the action of the hepatopancreas homogenate, triacylglycerols are converted into 1,2-diacylglycerol, and then into 2-monoacylglycerol and 1-monoacylglycerol. The 1-monoacylglycerol predominates in the reaction products. The use of NMR spectroscopy makes it possible to quickly detect hydrolysis products and evaluate the reaction direction.
