Expression profiles of immune-related genes in coelomocytes during regeneration after evisceration in Apostichopus japonicus

H F Dang; X Han; Y Guo; Q Li; S G Ye; J Liu; R J Li

Invertebrate Survival Journal (Jun 2020)

Expression profiles of immune-related genes in coelomocytes during regeneration after evisceration in Apostichopus japonicus

H F Dang,
X Han,
Y Guo,
Q Li,
S G Ye,
J Liu,
R J Li

Affiliations

H F Dang: Ministry of Agriculture and Rural Affairs Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Dalian Ocean University, Dalian 116023, China
X Han: Ministry of Agriculture and Rural Affairs Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Dalian Ocean University, Dalian 116023, China
Y Guo: Ministry of Agriculture and Rural Affairs Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Dalian Ocean University, Dalian 116023, China
Q Li: Department of Ocean Technology, College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng, 224051, China
S G Ye: Ministry of Agriculture and Rural Affairs Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Dalian Ocean University, Dalian 116023, China
J Liu: Ministry of Agriculture and Rural Affairs Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Dalian Ocean University, Dalian 116023, China
R J Li: Ministry of Agriculture and Rural Affairs Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Dalian Key Laboratory of Marine Animal Disease Control and Prevention, Dalian Ocean University, Dalian 116023, China

Abstract

Read online

A representative echinoderm, the sea cucumber Apostichopus japonicus has a special regeneration mechanism. The sea cucumber has no specific immune tissues or organs. Coelomocytes of sea cucumbers are involved in their cellular and humoral immunity. In this study, expression profiles of the main immune-related factors in sea cucumber coelomocytes were detected during coelomocyte regeneration after evisceration in A. japonicus. Immune-related factors Cu/Zn superoxide dismutase (Cu/Zn SOD), Catalase (CAT), C-type lysozyme (C-LYZ), Cathepsin D (CTSD), Melanotransferrin (MTF), Toll-like receptor (TLR), C-type lectin (C-LCT), Complement 3 (C3), Myeloid differentiation factor 88 (MYD88), Nuclear factor kappa-B (NF-κB), NF-κB inhibitor (IKB), TNF receptor-associated factor 6 (TRAF6), Peroxiredoxins (PRX), Nitric oxide synthase(NOS), Caspase-2 (CASP-2), Phenoloxidase (PO), and Glutathione Peroxidase(GPX), Heat shock protein 70 (HSP70) were detected by real-time fluorescent quantitative PCR at different time points during regeneration. The main immune-related genes in sea cucumber coelomocytes were significantly differentially expressed after evisceration, and an upregulation was observed for the majority of the considered genes. In summary, the discharge of viscera had a significant effect on expression of immune-related genes of sea cucumber coelomocytes. The expression level of each gene had a certain correlation with the sea cucumber regeneration process. The results provide reference data for the immune response of coelomocytes during regeneration.

Published in Invertebrate Survival Journal

ISSN: 1824-307X (Print)
Publisher: ISJ
Country of publisher: Italy
LCC subjects: Science: Biology (General)
Website: http://www.isj.unimo.it/

About the journal

Abstract

Keywords