Influence of blood donors' coagulation status in apheresis platelet aggregation in vitro

Abstract

Objective To investigate whether the blood donors' coagulation status may lead to apheresis platelet aggregation in vitro. Methods Thirty blood donors with aggregation in apheresis platelets collected by AMICUS blood cell separator no less than 3 times previously and occurred when the last time of apheresis donation were observed in aggregated group (referred to as the experimental group); Thirty donors without aggregation in apheresis platelets collected by AMICUS blood cell separator no less than 3 times were observed in the control group simultaneously. The basic platelet parameters in the two groups, including Plt, MPV, PDW, Pet, P-LCR were detected by automatic blood cell analyzer (BC-3000Plus), and thromboelastogram indexes including reaction time(R), kinetics time(K), kinetics of clot development(α), maximum amplitude (MA) and coagulation index(CI) were tested by Thrombosis elastography (TEG) before collection. With SPSS24.0 software, t test was used to compare the differences between the two groups. Results The CI value in experimental group was significantly different from that of the control group (0.48± 1.00 vs -0.99 ±1.96, P0.05 ) . Conclusion The coagulation status of blood donors may be an independent risk factor for the in vitro aggregation of apheresis platelets.

Keywords

• platelet aggregation in vitro
• coagulation status of blood donors
• apheresis platelet
• coagulation index(ci)
• platelet parameters
• blood cell separator