Journal of Laboratory Medicine (Oct 2021)

Clinical performance and potential of a SARS-CoV-2 detection kit without RNA purification steps

  • Sato Yuki,
  • Kondo Takashi,
  • Moriai Mikako,
  • Nirasawa Shinya,
  • Saeki Masachika,
  • Yakuwa Yuki,
  • Katayama Yuki,
  • Kitayama Ikumi,
  • Kobayashi Ryo,
  • Endoh Akemi,
  • Asanuma Koichi,
  • Fujiya Yoshihiro,
  • Takahashi Satoshi

DOI
https://doi.org/10.1515/labmed-2021-0073
Journal volume & issue
Vol. 45, no. 4-5
pp. 205 – 212

Abstract

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is rapidly spreading globally. Early diagnosis plays an essential role in controlling the infection. Therefore, early and accurate SARS-CoV-2 detection assays along with easy operation are required. The aim of this study was to compare the clinical performance of the Ampdirect™ 2019-nCoV Detection Kit (SHIMADZU assay), which does not require RNA purification steps, with that of the preexisting SARS-CoV-2 detection assays, which use a purified RNA template.

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