mSystems
(Oct 2021)
Assessing Multiplex Tiling PCR Sequencing Approaches for Detecting Genomic Variants of SARS-CoV-2 in Municipal Wastewater
Xuan Lin,
Melissa Glier,
Kevin Kuchinski,
Tenysha Ross-Van Mierlo,
David McVea,
John R. Tyson,
Natalie Prystajecky,
Ryan M. Ziels
Affiliations
Xuan Lin
Civil Engineering, The University of British Columbia, Vancouver, British Columbia, Canada
Melissa Glier
Environmental Microbiology, British Columbia Center for Disease Control Public Health Laboratory, Vancouver, British Columbia, Canada
Kevin Kuchinski
Environmental Microbiology, British Columbia Center for Disease Control Public Health Laboratory, Vancouver, British Columbia, Canada
Tenysha Ross-Van Mierlo
Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada
David McVea
Environmental Health Services, British Columbia Center for Disease Control Public Health Laboratory, Vancouver, British Columbia, Canada
John R. Tyson
Environmental Microbiology, British Columbia Center for Disease Control Public Health Laboratory, Vancouver, British Columbia, Canada
Natalie Prystajecky
Environmental Microbiology, British Columbia Center for Disease Control Public Health Laboratory, Vancouver, British Columbia, Canada
Ryan M. Ziels
ORCiD Civil Engineering, The University of British Columbia, Vancouver, British Columbia, Canada
DOI
https://doi.org/10.1128/mSystems.01068-21
Journal volume & issue
Vol. 6,
no. 5
Abstract
Read online
Monitoring the genomic characteristics of the SARS-CoV-2 virus circulating in a population can shed important insights into epidemiological aspects of the COVID-19 outbreak. Sequencing every clinical patient sample in a highly populous area is a difficult feat, and thus sequencing SARS-CoV-2 RNA in municipal wastewater offers great promise to augment genomic surveillance by characterizing a pooled population sample matrix, particularly during an escalating outbreak.
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