Molecular characterisation of Zucchini yellow mosaic virus infecting Cucurbita pepo in Egypt

Abstract

The complete nucleotide sequence of Zucchini yellow mosaic virus isolate from Egypt (ZYMV-Egz_MT383108) was determined. The sequence comparisons suggested that the isolate belongs to Group A. The sequence analysis of the Egyptian isolate showed the highest similarity (~96-97%) with the isolates leaf1 (KJ923767.1) and PA_2006 (JQ716413.1) from the USA and the lowest similarity (84%) with an isolate (AF014811.2) from Singapore. The phylogenetic analysis revealed that ZYMV-Egz occupied a distinct clade together with the USA isolates in Group A, known to be the most widespread throughout the world. This is a first record of the complete nucleotide sequence of an Egyptian isolate of ZYMV.

Keywords

• cucurbits
• genome sequence
• phylogenetic analysis
• rt-pcr
• virus identification