In Vitro Germination and Organogenesis of Endangered Neo-Endemic Baltic Dunes Species <i>Linaria loeselii</i> Schweigg
Lidia Banaszczyk,
Michał D. Starke,
Damian Szelbracikowski,
Julia Ścibior,
Małgorzata Kapusta
Affiliations
Lidia Banaszczyk
Laboratory of Plant Cytology and Embryology, Department of Plant Experimental Biology and Biotechnology, Faculty of Biology, University of Gdańsk, 59 Wita Stwosza St., 80-308 Gdańsk, Poland
Michał D. Starke
Laboratory of Plant Cytology and Embryology, Department of Plant Experimental Biology and Biotechnology, Faculty of Biology, University of Gdańsk, 59 Wita Stwosza St., 80-308 Gdańsk, Poland
Damian Szelbracikowski
Student Research Group of Biotechnology and Embryology “Explantatus”, Faculty of Biology, University of Gdańsk, 59 Wita Stwosza St., 80-308 Gdańsk, Poland
Julia Ścibior
Student Research Group of Biotechnology and Embryology “Explantatus”, Faculty of Biology, University of Gdańsk, 59 Wita Stwosza St., 80-308 Gdańsk, Poland
Małgorzata Kapusta
Student Research Group of Biotechnology and Embryology “Explantatus”, Faculty of Biology, University of Gdańsk, 59 Wita Stwosza St., 80-308 Gdańsk, Poland
This study focuses on the endangered neo-endemic Baltic dunes species Linaria loeselii Schweigg. (Plantaginaceae), also known as Linaria odora (M. Bieb.). By utilizing in vitro cultures, we successfully germinated seeds collected in situ. Our method, which involved using media supplemented with 5 µmol/L 6-benzylaminopurine, led to the indirect regeneration of shoots after 60 days of culture in the dark, significantly increasing the number of progeny plants. Additionally, the medium supplemented with 2.85 μmol/L indole-3-acetic acid and 10.2 μmol/L paclobutrazol allowed rooting after 30 days of shoot fragments. This research provides a potential basis for developing Linaria loeselii introduction programs into the environment, thereby contributing to the conservation of this endangered species.
