Detection of Guanine and Adenine Using an Aminated Reduced Graphene Oxide Functional Membrane-Modified Glassy Carbon Electrode
Di Li,
Xiao-Lu Yang,
Bao-Lin Xiao,
Fang-Yong Geng,
Jun Hong,
Nader Sheibani,
Ali Akbar Moosavi-Movahedi
Affiliations
Di Li
School of Life Sciences, Henan University, JinMing Road, Kaifeng 475000, China
Xiao-Lu Yang
School of Life Sciences, Henan University, JinMing Road, Kaifeng 475000, China
Bao-Lin Xiao
School of Life Sciences, Henan University, JinMing Road, Kaifeng 475000, China
Fang-Yong Geng
School of Life Sciences, Henan University, JinMing Road, Kaifeng 475000, China
Jun Hong
School of Life Sciences, Henan University, JinMing Road, Kaifeng 475000, China
Nader Sheibani
Department of Ophthalmology and Visual Sciences and Biomedical Engineering, University of Wisconsin School of Medicine and Public Health, Madison, WI 53726, USA
Ali Akbar Moosavi-Movahedi
Institute of Biochemistry and Biophysics, University of Tehran, Enquelab Avenue, Tehran 13145-1384, Iran
A new electrochemical sensor based on a Nafion, aminated reduced graphene oxide and chitosan functional membrane-modified glassy carbon electrode was proposed for the simultaneous detection of adenine and guanine. Fourier transform-infrared spectrometry (FTIR), transmission electron microscopy (TEM), and electrochemical methods were utilized for the additional characterization of the membrane materials. The prepared electrode was utilized for the detection of guanine (G) and adenine (A). The anodic peak currents to G and A were linear in the concentrations ranging from 0.1 to 120 μM and 0.2 to 110 μM, respectively. The detection limits were found to be 0.1 μM and 0.2 μM, respectively. Moreover, the modified electrode could also be used to determine G and A in calf thymus DNA.
