Induction of Skeletal Muscle Injury by Intramuscular Injection of Cardiotoxin in Mouse
Xin Fu,
Sheng Li,
Minzhi Jia,
Wenjun Yang,
Ping Hu
Affiliations
Xin Fu
Spine Center, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
Sheng Li
Spine Center, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
Minzhi Jia
State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China
Wenjun Yang
Spine Center, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
Ping Hu
Spine Center, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, ChinaGuangzhou Laboratory, No. 9 XingDaoHuan Road, Guangzhou International Bio lsland, Guangzhou 510005, China, Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China
Skeletal muscle is the most abundant tissue in the human body and has a tremendous capability to regenerate in response to muscle injuries and diseases. Induction of acute muscle injury is a common method to study muscle regeneration in vivo. Cardiotoxin (CTX) belongs to the family of snake venom toxins and is one of the most common reagents to induce muscle injury. Intramuscular injection of CTX causes overwhelming muscle contraction and lysis of myofibers. The induced acute muscle injury triggers muscle regeneration, allowing in-depth studies on muscle regeneration. This protocol describes a detailed procedure of intramuscular injection of CTX to induce acute muscle injury that could be also applied in other mammalian models.
