Oxidative Modification Status of Human Serum Albumin Caused by Chronic Low-Dose Radiation Exposure in Mamuju, Sulawesi, Indonesia
Masaru Yamaguchi,
Yota Tatara,
Eka Djatnika Nugraha,
Yuki Tamakuma,
Yoshiaki Sato,
Tomisato Miura,
Masahiro Hosoda,
Shinji Yoshinaga,
Mukh Syaifudin,
Shinji Tokonami,
Ikuo Kashiwakura
Affiliations
Masaru Yamaguchi
Graduate School of Health Sciences, Hirosaki University, 66-1 Hon-cho, Hirosaki 036-8564, Japan
Yota Tatara
Graduate School of Medicine, Hirosaki University, 5 Zaifu-cho, Hirosaki 036-8562, Japan
Eka Djatnika Nugraha
The Research Center for Safety, Metrology, and Nuclear Quality Technology, National Research and Innovation Agency of Indonesia, JI. Lebak Bulus Raya No. 49, Jakarta Selatan 12440, Indonesia
Yuki Tamakuma
Center for Radiation Research and Education, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
Yoshiaki Sato
Graduate School of Health Sciences, Hirosaki University, 66-1 Hon-cho, Hirosaki 036-8564, Japan
Tomisato Miura
Institute of Radiation Emergency Medicine, Hirosaki University, 66-1 Hon-cho, Hirosaki 036-8564, Japan
Masahiro Hosoda
Graduate School of Health Sciences, Hirosaki University, 66-1 Hon-cho, Hirosaki 036-8564, Japan
Shinji Yoshinaga
Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku 734-8553, Japan
Mukh Syaifudin
The Research Center for Radioisotope, Radiopharmaceutical and Biodosimetry Technology, Research Organization for Nuclear Energy, National Research and Innovation Agency, Building 71, Kawasan Puspiptek, Setu, Tangerang Selatan 15310, Indonesia
Shinji Tokonami
Institute of Radiation Emergency Medicine, Hirosaki University, 66-1 Hon-cho, Hirosaki 036-8564, Japan
Ikuo Kashiwakura
Graduate School of Health Sciences, Hirosaki University, 66-1 Hon-cho, Hirosaki 036-8564, Japan
The recently discovered high-level natural background radiation area (HBRA) of Mamuju in Indonesia provides a unique opportunity to study the biological effects of chronic low-dose radiation exposure on a human population. The mean total effective dose in the HBRA was approximately 69.6 mSv y−1 (range: 47.1 to 115.2 mSv y−1), based on a re-evaluation of the individual radiation exposure dose; therefore, proteomic analyses of serum components and oxidative modification profiling of residents living in the HBRA were reconducted using liquid chromatography-tandem mass spectrometry. The analysis of the oxidative modification sequences of human serum albumin revealed significant moderate correlations between the radiation dose and the modification of 12 sequences, especially the 111th methionine, 162nd tyrosine, 356th tyrosine, and 470th methionine residues. In addition, a dose-dependent variation in 15 proteins of the serum components was detected in the serum of residents exposed to chronic low-dose radiation. These findings suggest that the alterations in the expression of specific proteins and the oxidative modification responses of serum albumin found in exposed humans may be important indicators for considering the effects of chronic low-dose radiation exposure on living organisms, implying their potential utility as biomarkers of radiation dose estimation.
