PLoS ONE (Jan 2011)

Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.

  • Paolo Annibale,
  • Stefano Vanni,
  • Marco Scarselli,
  • Ursula Rothlisberger,
  • Aleksandra Radenovic

DOI
https://doi.org/10.1371/journal.pone.0022678
Journal volume & issue
Vol. 6, no. 7
p. e22678

Abstract

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In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.