Frontiers in Cell and Developmental Biology (Nov 2022)
The diversity of trophoblast cells and niches of placenta accreta spectrum disorders revealed by single-cell RNA sequencing
- Jingmei Ma,
- Jingmei Ma,
- Jingmei Ma,
- Yawei Liu,
- Yawei Liu,
- Yawei Liu,
- Yawei Liu,
- Yawei Liu,
- Zhirong Guo,
- Zhirong Guo,
- Run Sun,
- Run Sun,
- Run Sun,
- Run Sun,
- Xinrui Yang,
- Xinrui Yang,
- Weiran Zheng,
- Weiran Zheng,
- Yongdan Ma,
- Yongdan Ma,
- Yin Rong,
- Yin Rong,
- Yin Rong,
- Yin Rong,
- Hongmei Wang,
- Hongmei Wang,
- Hongmei Wang,
- Hongmei Wang,
- Huixia Yang,
- Huixia Yang,
- Zhenyu Xiao,
- Zhenyu Xiao,
- Zhenyu Xiao,
- Zhenyu Xiao,
- Zhenyu Xiao
Affiliations
- Jingmei Ma
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Jingmei Ma
- Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China
- Jingmei Ma
- Beijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus, Beijing, China
- Yawei Liu
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Yawei Liu
- Innovation Academy for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Yawei Liu
- Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Yawei Liu
- University of Chinese Academy of Sciences, Beijing, China
- Yawei Liu
- Dushu Lake Hospital Affiliated to Soochow University, Medical Center of Soochow University, Suzhou Dushu Lake Hospital, Suzhou, China
- Zhirong Guo
- Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China
- Zhirong Guo
- Beijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus, Beijing, China
- Run Sun
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Run Sun
- Innovation Academy for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Run Sun
- Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Run Sun
- University of Chinese Academy of Sciences, Beijing, China
- Xinrui Yang
- Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China
- Xinrui Yang
- Beijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus, Beijing, China
- Weiran Zheng
- Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China
- Weiran Zheng
- Beijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus, Beijing, China
- Yongdan Ma
- Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China
- Yongdan Ma
- Beijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus, Beijing, China
- Yin Rong
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Yin Rong
- Innovation Academy for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Yin Rong
- Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Yin Rong
- University of Chinese Academy of Sciences, Beijing, China
- Hongmei Wang
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Hongmei Wang
- Innovation Academy for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Hongmei Wang
- Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Hongmei Wang
- University of Chinese Academy of Sciences, Beijing, China
- Huixia Yang
- Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China
- Huixia Yang
- Beijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus, Beijing, China
- Zhenyu Xiao
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
- Zhenyu Xiao
- Innovation Academy for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Zhenyu Xiao
- Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China
- Zhenyu Xiao
- University of Chinese Academy of Sciences, Beijing, China
- Zhenyu Xiao
- School of Life Science, Beijing Institute of Technology, Beijing, China
- DOI
- https://doi.org/10.3389/fcell.2022.1044198
- Journal volume & issue
-
Vol. 10
Abstract
Placenta accreta spectrum disorders (PAS) are severe pregnancy complications that occur when extravillous trophoblast cells (EVTs) invade beyond the uterine inner myometrium and are characterized by hypervascularity on prenatal ultrasound and catastrophic postpartum hemorrhage. The potential mechanisms remain incompletely understood. With single-cell RNA-sequencing analysis on the representative invasive parts and the normal part obtained from the same PAS placenta, we profiled the pathological landscape of invasive PAS placenta and deciphered an intensified differentiation pathway from progenitor cytotrophoblasts (CTBs) to EVTs via LAMB4+ and KRT6A+ CTBs. In the absence of the decidua, the invasive trophoblasts of various differentiation states interacted with ADIRF+ and DES+ maternal stromal cells. The PAS-associated hypervascularity might be due to the enhanced crosstalk of trophoblasts, stromal cells and vascular endothelial cells. Finally, we presented an immune microenvironmental landscape of invasive PAS. The pathogenesis of PAS could be further explored with current resources for future targeted translational studies.
Keywords
