Uterine lavage microbiological composition of dairy subclinical endometritis

Abstract

The article presents the data on the dairy cows’ uterine lavage microbial composition characteristics suffering from subclinical endometritis. Subclinical endometritis is accompanied by the normal microbiota composition changes in the cow uterus and the disease-causing and opportunistic microorganisms’ growth. To perform uterine contents lavage, polystyrene disposable pipettes connected to a 50 ml disposable syringe using a plastic adapter were used. Subclinical endometritis was diagnosed in 22 cows (17.19%). Sixty-one dairy cows out of 128 (47.66%) were healthy. Microorganisms of the Enterobacteriaceae family: E. coli, P. vulgaris, S. enteritidis, pathogenic streptococci: S. agalactiae, S. pyogenes and staphylococci: S. aureus, S. epidermidis were selected. The total microbial contamination of the lavage samples was 64.72±8.80 CFU/ml. The most common microorganisms were microorganisms of the Enterobacteriaceae family: E. coli, S. enteritidis and P. vulgaris with 2069 CFU /ml. Pathogenic streptococci amounted to 1989 CFU /ml and staphylococci to 1,579 CFU /ml.