4D Visualization of replication foci in mammalian cells corresponding to individual replicons

V. O. Chagin; C. S. Casas-Delucchi; M. Reinhart; L. Schermelleh; Y. Markaki; A. Maiser; J. J. Bolius; A. Bensimon; M. Fillies; P. Domaing; Y. M. Rozanov; H. Leonhardt; M. C. Cardoso

doi:10.1038/ncomms11231

Nature Communications (Apr 2016)

4D Visualization of replication foci in mammalian cells corresponding to individual replicons

V. O. Chagin,
C. S. Casas-Delucchi,
M. Reinhart,
L. Schermelleh,
Y. Markaki,
A. Maiser,
J. J. Bolius,
A. Bensimon,
M. Fillies,
P. Domaing,
Y. M. Rozanov,
H. Leonhardt,
M. C. Cardoso

Affiliations

V. O. Chagin: Department of Biology, Technische Universitaet Darmstadt
C. S. Casas-Delucchi: Department of Biology, Technische Universitaet Darmstadt
M. Reinhart: Department of Biology, Technische Universitaet Darmstadt
L. Schermelleh: Department of Biochemistry, Micron Advanced Bioimaging Unit, University of Oxford
Y. Markaki: Center for Integrated Protein Science at the Department of Biology, Ludwig Maximilians University Munich
A. Maiser: Center for Integrated Protein Science at the Department of Biology, Ludwig Maximilians University Munich
J. J. Bolius: Department of Biology, Technische Universitaet Darmstadt
A. Bensimon: Genomic Vision
M. Fillies: Max Delbrueck Center for Molecular Medicine
P. Domaing: Max Delbrueck Center for Molecular Medicine
Y. M. Rozanov: Laboratory of chromosome stability, Institute of Cytology
H. Leonhardt: Center for Integrated Protein Science at the Department of Biology, Ludwig Maximilians University Munich
M. C. Cardoso: Department of Biology, Technische Universitaet Darmstadt

DOI: https://doi.org/10.1038/ncomms11231
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 12

Abstract

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Whether replication happens at individual replicons or in replication factories is a controversial debate. Here the authors use super-resolution microscopy and analysis of replication fork speed to present evidence in favour of replicons.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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