DEPS-1 is required for piRNA-dependent silencing and PIWI condensate organisation in Caenorhabditis elegans

Kin Man Suen; Fabian Braukmann; Richard Butler; Dalila Bensaddek; Alper Akay; Chi-Chuan Lin; Dovilė Milonaitytė; Neel Doshi; Alexandra Sapetschnig; Angus Lamond; John Edward Ladbury; Eric Alexander Miska

doi:10.1038/s41467-020-18089-1

Nature Communications (Aug 2020)

DEPS-1 is required for piRNA-dependent silencing and PIWI condensate organisation in Caenorhabditis elegans

Kin Man Suen,
Fabian Braukmann,
Richard Butler,
Dalila Bensaddek,
Alper Akay,
Chi-Chuan Lin,
Dovilė Milonaitytė,
Neel Doshi,
Alexandra Sapetschnig,
Angus Lamond,
John Edward Ladbury,
Eric Alexander Miska

Affiliations

Kin Man Suen: Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge
Fabian Braukmann: Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge
Richard Butler: Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge
Dalila Bensaddek: Laboratory for Quantitative Proteomics, Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee
Alper Akay: Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge
Chi-Chuan Lin: School of Molecular and Cellular Biology, University of Leeds, LC Miall Building
Dovilė Milonaitytė: School of Molecular and Cellular Biology, University of Leeds, LC Miall Building
Neel Doshi: University of Cambridge, School of Clinical Medicine, Cambridge Biomedical Campus
Alexandra Sapetschnig: Wellcome Sanger Institute, Wellcome Trust Genome Campus
Angus Lamond: Laboratory for Quantitative Proteomics, Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee
John Edward Ladbury: School of Molecular and Cellular Biology, University of Leeds, LC Miall Building
Eric Alexander Miska: Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge

DOI: https://doi.org/10.1038/s41467-020-18089-1
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

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The C. elegans PIWI protein PRG-1 resides in the membraneless organelle P granule. Here, the authors identify the constitutive P granule protein DEPS-1 as an interactor of PRG-1 and show its function in piRNA-dependent silencing. DEPS-1 and PRG-1 form elongated condensates in vivo.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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