Molecular epidemiological typing of M. tuberculosis isolates isolated from Turkey's Eastern Anatolia with in house PCR method

Abstract

Purpose: The aim of this study as to compare microscopy and culture results of samples, determine drug resistance rates of the isolates, evaluate epidemiological relationship between the strains with ERIC-PCR, RAPD-PCR, OUT-PCR based on in house PCR technique. Materials and Methods: Direct microscopy and culture results of 2010 samples were analyzed. Drug sensitivity results were obtained from TULSA. The typing of isolates based on in house PCR was carried out in the microbiology laboratory of a faculty of medicine in a state university. Results: Of positive samples, 2.68% had Acid-resistant bacilli (ARB) positive + culture positivity, 2.93% had ARB positive and culture negative in smear, and 1.34% had ARB negative and culture positivity in smear. Resistance to primary antituberculous (anti-TB) drugs wasn’t observed in 33 culture positive isolates, whereas resistance to one or more primary anti-TB drugs was observed in 9.09%. Single drug resistance was 3.03%, resistance to Isoniazid (INH) and INH critical drugs was 6.06%. Isolates were divided 3 groups by ERIC-PCR, 5 groups for OUT-PCR and 6 by RAPD-PCR. Conclusion: Positivity rates were low due to low rate of studied samples, negative samples taken during treatment process. Molecular techniques like ERIC-PCR, RAPD-PCR and OUT-PCR are easy, fast and inexpensive methods for the epidemiological typing of Mycobacterium tuberculosis (MTB) in evaluating distinctions, similarities between origins.

Keywords

• mycobacterium tuberculosis
• ezn
• eric-pcr
• rapd-pcr
• out-pcr
• mycobacterium tuberculosis
• ezn
• eric-pcr
• rapd-pcr
• out-pcr