International Journal of General Medicine (Jan 2022)

The Association of TNF-α Promoter Polymorphisms with Genetic Susceptibility to Cervical Cancer in a Chinese Han Population

  • Yang J,
  • Wang Y,
  • Zhang S,
  • Li Y,
  • Li C,
  • Liu W,
  • Liu S,
  • Liang Y,
  • Zhang X,
  • Yan Z,
  • Shi L,
  • Yao Y

Journal volume & issue
Vol. Volume 15
pp. 417 – 427

Abstract

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Jia Yang,1,* Yingying Wang,2,* Shao Zhang,3 Yu Li,4 Chuanyin Li,1 Weipeng Liu,1 Shuyuan Liu,1 Yan Liang,1 Xinwen Zhang,1 Zhiling Yan,3 Li Shi,1 Yufeng Yao1 1Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan, 650118, People’s Republic of China; 2School of Basic Medical Science, Kunming Medical University, Kunming, 650500, People’s Republic of China; 3Department of Gynaecologic Oncology, The No. 3 Affiliated Hospital of Kunming Medical University, Kunming, 650118, People’s Republic of China; 4Department of Obstetrics, The No. 1 People’s Hospital of Kunming, Kunming, 650011, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yufeng YaoInstitute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, 650118, Yunnan, People’s Republic of ChinaEmail [email protected]; [email protected]: The tumour necrosis factor-α (TNF-α) gene plays an important role in the host immune response, which will influence the development and clearance of cancer. Polymorphism of the TNF-α promoter region is considered to influence its transcription and be a risk factor for tumorigenesis. In the current study, we evaluated the role of TNF-α promoter region polymorphisms in susceptibility to cervical intraepithelial neoplasia (CIN) and cervical cancer (CC).Methods: A total of 2732 subjects, including 1173 healthy controls, 579 patients with CIN and 980 patients with CC in a Chinese Han population, were selected for the current study. Five SNPs in the TNF-α promoter, rs1799964 (− 1031 C>T), rs1800630 (− 863 A>C), rs1799724 (− 857 C>T), rs1800629 (− 308 A>G) and rs361525 (− 238 A>G), were selected and genotyped using TaqMan Assays. The association of these SNPs with CIN and cervical cancer was evaluated among healthy controls, CIN and CC patients.Results: The frequency distribution of rs1800629 and rs361525 alleles was significantly different between the CC group and the control group (P=0.009 and P=0.002). The rs1800629 A allele was found to be a protective factor for CC (OR=0.72; 95% CI=0.56– 0.92). The rs361525 A allele was found to be a risk factor for CC (OR=1.69; 95% CI=1.21– 2.38). After pathological subtyping of CC, the allele distribution of rs1800629 and rs361525 were both significantly different between the cervical squamous cell carcinoma and control groups (P=0.002 and P< 0.001). The rs1800629 A allele was protective factor for cervical squamous cell carcinoma (OR=0.66; 95% CI=0.50– 0.86). The rs361525 A allele was a risk factor for cervical squamous cell carcinoma (OR=1.87; 95% CI=1.32– 2.65). Moreover, the genotypic frequency of rs361525 was significantly different between cervical cancer stage I and stage II (P=0.003).Conclusion: The rs1800629 and rs361525 in the TNF-α promoter are associated with susceptibility to CC in the Chinese Han population.Keywords: cervical intraepithelial neoplasia, CIN, cervical cancer, CC, promoter, single-nucleotide polymorphism, SNP, tumour necrosis factor-α, TNF-α

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