Antibiotics (Dec 2024)
High-Risk Lineages of Hybrid Plasmids Carrying Virulence and Carbapenemase Genes
- Valeria V. Shapovalova,
- Polina S. Chulkova,
- Vladimir A. Ageevets,
- Varvara Nurmukanova,
- Irina V. Verentsova,
- Asya A. Girina,
- Irina N. Protasova,
- Victoria S. Bezbido,
- Victor I. Sergevnin,
- Irina V. Feldblum,
- Larisa G. Kudryavtseva,
- Sergey N. Sharafan,
- Vladislav V. Semerikov,
- Marina L. Babushkina,
- Inna R. Valiullina,
- Nikita S. Chumarev,
- Guzel S. Isaeva,
- Natalya A. Belyanina,
- Irina U. Shirokova,
- Tatiana M. Mrugova,
- Elena I. Belkova,
- Svetlana D. Artemuk,
- Aleksandra A. Meltser,
- Marina V. Smirnova,
- Tatyana N. Akkonen,
- Nataliya A. Golovshchikova,
- Oleg V. Goloshchapov,
- Alexey B. Chukhlovin,
- Lubov N. Popenko,
- Elena Y. Zenevich,
- Aleksandr A. Vlasov,
- Galina V. Mitroshina,
- Marina S. Bordacheva,
- Irina V. Ageevets,
- Ofeliia S. Sulian,
- Alisa A. Avdeeva,
- Vladimir V. Gostev,
- Irina A. Tsvetkova,
- Maria A. Yakunina,
- Ekaterina U. Vasileva,
- Alina D. Matsvay,
- Dmitry I. Danilov,
- Yulia A. Savochkina,
- German A. Shipulin,
- Sergey V. Sidorenko
Affiliations
- Valeria V. Shapovalova
- Centre for Strategic Planning, of the Federal Medical and Biological Agency, Moscow 119121, Russia
- Polina S. Chulkova
- Pediatric Research and Clinical Center for Infectious Diseases, Saint Petersburg 197022, Russia
- Vladimir A. Ageevets
- Pediatric Research and Clinical Center for Infectious Diseases, Saint Petersburg 197022, Russia
- Varvara Nurmukanova
- Centre for Strategic Planning, of the Federal Medical and Biological Agency, Moscow 119121, Russia
- Irina V. Verentsova
- Khanty-Mansyisk Regional Clinical Hospital, Khanty-Mansiysk 622018, Russia
- Asya A. Girina
- Khanty-Mansiysk State Medical Academy, Khanty-Mansiysk 628011, Russia
- Irina N. Protasova
- Department of Microbiology Named After Associate Professor B.M. Zelmanovich, Krasnoyarsk State Medical University Named After Professor V. F. Voyno-Yasenetsky, Krasnoyarsk 660022, Russia
- Victoria S. Bezbido
- Krasnoyarsk Interdistrict Clinical Emergency Hospital Named After N.S. Karpovich, Krasnoyarsk 660062, Russia
- Victor I. Sergevnin
- Department of Epidemiology and Hygiene, Perm State Medical University Named After Academician E.A. Wagner, Perm 614000, Russia
- Irina V. Feldblum
- Department of Epidemiology and Hygiene, Perm State Medical University Named After Academician E.A. Wagner, Perm 614000, Russia
- Larisa G. Kudryavtseva
- Federal Center for Cardiovascular Surgery Named After S.G. Sukhanov of the Ministry of Health of the Russian Federation, Perm 614013, Russia
- Sergey N. Sharafan
- Federal Center for Cardiovascular Surgery Named After S.G. Sukhanov of the Ministry of Health of the Russian Federation, Perm 614013, Russia
- Vladislav V. Semerikov
- State Budgetary Healthcare Institution of Perm Krai «Perm Regional Clinical Infectious Diseases Hospital», Perm 614014, Russia
- Marina L. Babushkina
- State Budgetary Healthcare Institution of Perm Krai «Perm Regional Clinical Infectious Diseases Hospital», Perm 614014, Russia
- Inna R. Valiullina
- Republican Clinical Hospital of the Ministry of Health of the Republic of Tatarstan, Kazan 420064, Russia
- Nikita S. Chumarev
- Department of Microbiology Named After Academician V.M. Aristovsky, Kazan State Medical University of the Ministry of Health of the Russian Federation, Kazan 420012, Russia
- Guzel S. Isaeva
- Department of Microbiology Named After Academician V.M. Aristovsky, Kazan State Medical University of the Ministry of Health of the Russian Federation, Kazan 420012, Russia
- Natalya A. Belyanina
- Department of Epidemiology, Microbiology and Evidence-Based Medicine, Federal State Budgetary Educational Institution of Higher Education «Privolzhsky Research Medical University» of the Ministry of Health of the Russian Federation (FSBEI HE PRMU MOH Russia), Nizhny Novgorod 603005, Russia
- Irina U. Shirokova
- Department of Epidemiology, Microbiology and Evidence-Based Medicine, Federal State Budgetary Educational Institution of Higher Education «Privolzhsky Research Medical University» of the Ministry of Health of the Russian Federation (FSBEI HE PRMU MOH Russia), Nizhny Novgorod 603005, Russia
- Tatiana M. Mrugova
- State Budgetary Institution of Healthcare of the City of Moscow «Moscow Science and Practical Centre for the Laboratory Research of the Department of Healthcare of the City of Moscow», Moscow 127015, Russia
- Elena I. Belkova
- Mariinsky Hospital, Saint Petersburg 191014, Russia
- Svetlana D. Artemuk
- Mariinsky Hospital, Saint Petersburg 191014, Russia
- Aleksandra A. Meltser
- Mariinsky Hospital, Saint Petersburg 191014, Russia
- Marina V. Smirnova
- Mariinsky Hospital, Saint Petersburg 191014, Russia
- Tatyana N. Akkonen
- City Polyclinic No. 74, Saint Petersburg 197762, Russia
- Nataliya A. Golovshchikova
- City Polyclinic No. 74, Saint Petersburg 197762, Russia
- Oleg V. Goloshchapov
- R. M. Gorbacheva Memorial Institute of Oncology, Hematology and Transplantation, Pavlov University, Saint Petersburg 197022, Russia
- Alexey B. Chukhlovin
- R. M. Gorbacheva Memorial Institute of Oncology, Hematology and Transplantation, Pavlov University, Saint Petersburg 197022, Russia
- Lubov N. Popenko
- I. I. Dzhanelidze Research Institute of Emergency Medical Service, Saint Petersburg 192242, Russia
- Elena Y. Zenevich
- Alexander Hospital, Saint Petersburg 190068, Russia
- Aleksandr A. Vlasov
- Psychiatric Hospital No. 1 Named After P. P. Kaschenko, Saint Petersburg 195009, Russia
- Galina V. Mitroshina
- Napalkov State Budgetary Healthcare Institution “Saint-Petersburg Clinical Scientific and Practical Center for Specialised Types of Medical Care (Oncological)”, Saint Petersburg 197101, Russia
- Marina S. Bordacheva
- Napalkov State Budgetary Healthcare Institution “Saint-Petersburg Clinical Scientific and Practical Center for Specialised Types of Medical Care (Oncological)”, Saint Petersburg 197101, Russia
- Irina V. Ageevets
- Pediatric Research and Clinical Center for Infectious Diseases, Saint Petersburg 197022, Russia
- Ofeliia S. Sulian
- Pediatric Research and Clinical Center for Infectious Diseases, Saint Petersburg 197022, Russia
- Alisa A. Avdeeva
- Pediatric Research and Clinical Center for Infectious Diseases, Saint Petersburg 197022, Russia
- Vladimir V. Gostev
- Pediatric Research and Clinical Center for Infectious Diseases, Saint Petersburg 197022, Russia
- Irina A. Tsvetkova
- Pediatric Research and Clinical Center for Infectious Diseases, Saint Petersburg 197022, Russia
- Maria A. Yakunina
- Murmansk Regional Clinical-Hospital Named After P.A. Bayandin, Murmasnk 183047, Russia
- Ekaterina U. Vasileva
- Murmansk Regional Clinical-Hospital Named After P.A. Bayandin, Murmasnk 183047, Russia
- Alina D. Matsvay
- Centre for Strategic Planning, of the Federal Medical and Biological Agency, Moscow 119121, Russia
- Dmitry I. Danilov
- Centre for Strategic Planning, of the Federal Medical and Biological Agency, Moscow 119121, Russia
- Yulia A. Savochkina
- Centre for Strategic Planning, of the Federal Medical and Biological Agency, Moscow 119121, Russia
- German A. Shipulin
- Centre for Strategic Planning, of the Federal Medical and Biological Agency, Moscow 119121, Russia
- Sergey V. Sidorenko
- Pediatric Research and Clinical Center for Infectious Diseases, Saint Petersburg 197022, Russia
- DOI
- https://doi.org/10.3390/antibiotics13121224
- Journal volume & issue
-
Vol. 13,
no. 12
p. 1224
Abstract
Background/Objectives: Carbapenem-resistant Enterobacterales (CRE) are a global health threat due to their high morbidity and mortality rates and limited treatment options. This study examines the plasmid-mediated transmission of virulence and antibiotic resistance determinants in carbapenem-resistant Klebsiella pneumoniae (Kpn) and Escherichia coli (E. coli) isolated from Russian hospitals. Methods: We performed short- and long-read whole-genome sequencing of 53 clinical isolates (48 Kpn and 5 E. coli) attributed to 15 genetic lineages and collected from 21 hospitals across nine Russian cities between 2016 and 2022. Results: The plasmid analysis identified 18 clusters that showed high concordance with replicon typing, with all clusters having a major replicon type. The majority of plasmids in the IncHI1B(pNDM-MAR)/IncFIB(pNDM-Mar)-like cluster (79.16%) carried both antibiotic resistance genes (e.g., blaNDM-1 and blaOXA-48) and virulence factors (VFs) such as siderophore genes. We hypothesized that hybrid plasmids could play a critical role in the dissemination of antibiotic resistance genes and VFs. Comparative analyses with global plasmid databases revealed high-risk lineages of hybrid plasmids that are predominantly spread throughout Russia at present. Conclusions: Our findings underscore the importance of monitoring plasmid backbones for clinical management, surveillance, and infection control activities.
Keywords
