Bio-Protocol (May 2017)

Imaging the Pharynx to Measure the Uptake of Doxorubicin in Caenorhabditis elegans

  • Sivathevy Amirthagunabalasingam,
  • Arturo Papaluca,
  • Taramatti Harihar,
  • Dindial Ramotar

DOI
https://doi.org/10.21769/bioprotoc.2291
Journal volume & issue
Vol. 7, no. 10

Abstract

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Caenorhabditis elegans offers an array of advantages to investigate the roles of uptake transporters. Herein, an epifluorescent microscopy approach was developed to monitor the uptake of the autofluorescent anticancer drug, doxorubicin, into the pharynx of C. elegans by organic cation transporters.