Rapid diagnosis of latent and active pulmonary tuberculosis by autofluorescence spectroscopy of blood plasma combined with artificial neural network algorithm

Abstract

The existing clinical diagnostic methods of pulmonary tuberculosis (TB) usually have some of the following limitations, such as time-consuming, invasive, radioactive, insufficiently sensitive and accurate. This study demonstrates the possibility of using blood plasma autofluorescence spectroscopy and Artificial Neural Network (ANN) algorithm for the rapid and accurate diagnosis of latent and active pulmonary TB from healthy subjects. The fluorescence spectra of blood plasma from 18 healthy volunteers, 12 individuals with latent TB infections and 80 active TB patients are measured and analyzed. By optimizing the ANN structure and activation functions, the ANN three-classification model achieves average classification accuracy of 96.3 %, and the accuracy of healthy persons, latent TB infections and active TB patients are 100 %, 83.3 % and 97.5 %, respectively, which is much better than the results of traditional Principal component analysis (PCA) and Linear discriminant analysis (LDA) method. To the best of our knowledge, this is the first research work of differentiating latent, active pulmonary TB cases from healthy samples with autofluorescence spectroscopy. As a rapid, accurate, safe, label-free, non-invasive and cost-effective technique, it can be developed as a promising diagnostic tool for the screening of pulmonary TB disease in the early stage.

Keywords

• Autofluorescence spectroscopy
• Blood plasma
• Pulmonary tuberculosis
• Artificial neural network
• Principal component analysis
• Linear discriminant analysis