Bio-Protocol (Feb 2013)

Measurement of Airway Responsiveness on Vigil and Unrestrained Mouse

  • François Daubeuf,
  • Laurent Reber,
  • Nelly Frossard

DOI
https://doi.org/10.21769/bioprotoc.328
Journal volume & issue
Vol. 3, no. 4

Abstract

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Airway hyperresponsiveness to methacholine is an important characteristic of asthma. This protocol describes how to measure airway response to methacholine in vigil and unrestraint mouse. The enhanced pause (PenH) is an index of the airway response to increasing doses of methacholine recorded by whole-body barometric plethysmography.