Molecular characterization and functional analysis of DMRT11E in black tiger shrimp (Penaeus monodon)

Wen-Ya Wei; Jian-Hua Huang; Qi-Bin Yang; Fa-Lin Zhou; Song Jiang; Yun-Dong Li; Shi-Gui Jiang; Li-Shi Yang

Aquaculture Reports (Feb 2022)

Molecular characterization and functional analysis of DMRT11E in black tiger shrimp (Penaeus monodon)

Wen-Ya Wei,
Jian-Hua Huang,
Qi-Bin Yang,
Fa-Lin Zhou,
Song Jiang,
Yun-Dong Li,
Shi-Gui Jiang,
Li-Shi Yang

Affiliations

Wen-Ya Wei: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, PR China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, PR China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China
Jian-Hua Huang: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, PR China; Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518108, PR China; Tropical Aquaculture Research and Development Center of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, PR China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China
Qi-Bin Yang: Tropical Aquaculture Research and Development Center of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, PR China
Fa-Lin Zhou: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, PR China; Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518108, PR China; Tropical Aquaculture Research and Development Center of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, PR China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China
Song Jiang: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, PR China; Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518108, PR China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China
Yun-Dong Li: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, PR China; Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518108, PR China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China
Shi-Gui Jiang: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, PR China; Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518108, PR China; Tropical Aquaculture Research and Development Center of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, PR China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China
Li-Shi Yang: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, PR China; Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518108, PR China; Tropical Aquaculture Research and Development Center of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, PR China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China; Correspondence to: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 231 Xingang West Road, Guangzhou 510300, PR China.

Journal volume & issue: Vol. 22
p. 100982

Abstract

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As a highly conserved transcription factor family, DMRTs (Doublesex and Mab-3-related transcription factors) are involved in sex determination and sexual differentiation in numerous animals. In the present study, we identified a DM (Doublesex/Mab-3)-domain gene in Penaeus monodon, which we named PmDMRT11E because it shared many similarities with DMRT11E homologs and has a close relationship in the phylogeny of different species. The three-dimensional structure and amino acid alignments indicated the stability and conservation of the DM domain. Real-time PCR analysis revealed that PmDMRT11E was highly and preferentially expressed in the testis of sub-adult shrimp. During embryogenesis, the expression level of PmDMRT11E was higher at the nauplius than at other periods. PmDMRT11E dsRNA injection significantly decreased the transcription factor Sox9 and increased ovarian marker Foxl2 expression, implying its important regulatory role in the mechanism of male determination. Moreover, dsDMRT11E caused abnormal sperm development, indicating that PmDMRT11E may be closely related to gonadal development and spermatogenesis.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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