Amelioration of type 2 diabetes by the novel 6, 8-guanidyl luteolin quinone-chromium coordination via biochemical mechanisms and gut microbiota interaction

Xiaodong Ge; Xiaoyu He; Junwei Liu; Feng Zeng; Ligen Chen; Wei Xu; Rong Shao; Ying Huang; Mohamed A. Farag; Esra Capanoglu; Hesham R. El-Seedi; Chao Zhao; Bin Liu

Journal of Advanced Research (Apr 2023)

Amelioration of type 2 diabetes by the novel 6, 8-guanidyl luteolin quinone-chromium coordination via biochemical mechanisms and gut microbiota interaction

Xiaodong Ge,
Xiaoyu He,
Junwei Liu,
Feng Zeng,
Ligen Chen,
Wei Xu,
Rong Shao,
Ying Huang,
Mohamed A. Farag,
Esra Capanoglu,
Hesham R. El-Seedi,
Chao Zhao,
Bin Liu

Affiliations

Xiaodong Ge: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
Xiaoyu He: National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
Junwei Liu: College of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China
Feng Zeng: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
Ligen Chen: College of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China
Wei Xu: College of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China
Rong Shao: College of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng, Jiangsu 224051, China
Ying Huang: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
Mohamed A. Farag: Pharmacognosy Department, College of Pharmacy, Cairo University, Cairo, Egypt
Esra Capanoglu: Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, Maslak 34469 Istanbul, Turkey
Hesham R. El-Seedi: Pharmacognosy Group, Department of Pharmaceutical Biosciences, BMC, Uppsala University, Uppsala, Box 591, SE 751 24 Uppsala, Sweden
Chao Zhao: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Corresponding authors at: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
Bin Liu: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Corresponding authors at: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.

Journal volume & issue: Vol. 46
pp. 173 – 188

Abstract

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Introduction: Luteolin is a plant-derived flavonoid that exhibits a broad range of pharmacological activities. Studies on luteolin have mainly focused on its use for hyperlipidaemia prevention, whereas the capacity of the flavonoid to hinder hyperglycaemia development remains underexplored. Objectives: To probe the anti-hyperglycemic mechanism of 6,8-guanidyl luteolin quinone-chromium coordination (GLQ.Cr), and to assess its regulatory effect on intestinal microbiota in type 2 diabetes mellitus (T2DM) mice. Methods: High-sucrose/high-fat diet-induced and intraperitoneal injection of streptozotocin was used to develop a T2DM model. Glycometabolism related indicators, histopathology, and gut microbiota composition in caecum samples were evaluated, and RNA sequencing (RNA-seq) of liver samples was conducted. Faecal microbiota transplantation (FMT) was further used to verify the anti-hyperglycemic activity of intestinal microbiota. Results: The administration of GLQ.Cr alleviated hyperglycaemia symptoms by improving liver and pancreatic functions and modulating gut microbe communities (Lactobacillus, Alistipes, Parabacteroides, Lachnoclostridium, and Desulfovibrio). RNA-seq analysis showed that GLQ.Cr mainly affected the peroxisome proliferative activated receptor (PPAR) signalling pathway in order to regulate abnormal glucose metabolism. FMT significantly modulated the abundance of Lactobacillus, Alloprevotella, Alistipes, Bacteroides, Ruminiclostridium, Brevundimonas and Pseudomonas in the caecum to balance blood glucose levels and counteract T2DM mice inflammation. Conclusion: GLQ.Cr improved the abnormal glucose metabolism in T2DM mice by regulating the PPAR signalling pathway and modulating intestinal microbial composition. FMT can improve the intestinal microecology of the recipient and in turn ameliorate the symptoms of T2DM-induced hyperglycaemia.

Published in Journal of Advanced Research

ISSN: 2090-1232 (Print); 2090-1224 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Science (General)
Website: http://www.journals.elsevier.com/journal-of-advanced-research/

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