Elasticity Changes in the Crystalline Lens during Oxidative Damage and the Antioxidant Effect of Alpha-Lipoic Acid Measured by Optical Coherence Elastography

Abstract

Age-related cataracts are one of the most prevalent causes of visual impairment around the world. Understanding the mechanisms of cataract development and progression is essential to enable early clinical diagnosis and treatment to preserve visual acuity. Reductive chemicals are potential medicines effective on cataract treatment. In this work, we investigated the cataract-induced oxidative damage in the crystalline lens and a kind of reductant, α-lipoic acid (ALA), ability to reduce the damage. We created oxidative environment to investigate the relationship between the progression of oxidative cataract and lenticular biomechanical properties measured by dynamic optical coherence elastography in porcine crystalline lenses ex vivo. The efficacy of ALA to minimize the stiffening of the lens was also quantified. The results showed a significant increase in Young’s modulus of the lens due to the formation of the oxidative cataract. We found a statistically significant difference between Young’s modulus of the lenses stored in phosphate-buffered saline and ALA solution after incubation in H2O2 solution for 3 h (43.0 ± 9.0 kPa versus 20.7 ± 3.5 kPa, respectively). These results show that the lens stiffness increases during oxidative cataract formation, and ALA has the potential to reverse stiffening of the lens caused by oxidative damage.

Keywords

• crystalline lens
• optical coherence elastography
• elasticity
• alpha-lipoic acid
• oxidation
• cataract