Frontiers in Nutrition (Nov 2022)

Bioassay-guided isolation of Fenghuang Dancong tea constituents with α-glucosidase inhibition activities

  • Hua Zhou,
  • Jin Liao,
  • Juanying Ou,
  • Jiayu Lin,
  • Jiayu Lin,
  • Jie Zheng,
  • Yifei Li,
  • Shiyi Ou,
  • Fu Liu

DOI
https://doi.org/10.3389/fnut.2022.1050614
Journal volume & issue
Vol. 9

Abstract

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An α-glucosidase inhibition assay showed the ethanolic extract of Fenghuang Dancong tea had potential α-glucosidase inhibitory activity. The most bioactive fraction, which was obtained via bioassay-guided isolation of the extract, was further purified to create five compounds, including three novel compounds (1–3). These compounds were analyzed and identified in detail using high-resolution-mass spectrometry and extensive one-dimensional and two-dimensional NMR spectroscopy experiments. Among the compounds, compound 1 contained cis double bonds and showed the strongest α-glucosidase inhibitory activity with an IC50 value of 7.51 μM, which is significantly lower than that of compound 2 with trans double bonds. Enzyme kinetic experiments showed that 1 was a reversible non-competitive α-glucosidase inhibitor.

Keywords