Jurnal MIPA (Nov 2014)

UJI TOKSISITAS DAN PERUBAHAN STRUKTUR MIKROANATOMI INSANG IKAN NILA LARASATI (Oreochromis nilloticus) YANG DIPAPAR TIMBAL ASETAT

  • FAM Mulyani,
  • P Widiyaningrum,
  • NR Utami

Journal volume & issue
Vol. 37, no. 1

Abstract

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Penelitian ini bertujuan untuk mengetahui Nilai LC50-96 jam pada uji toksisitas ikan Nila Larasati yang dipapar timbal; mengetahui perubahan struktur mikroanatomi dan mengetahui pada konsentrasi berapa timbal menyebabkan perubahan struktur mikroanatomi insang. Sampel uji toksisitas sebanyak 120 ekor untuk mengetahui tingkat kematian ikan hingga 50% dalam 96 jam, dan uji perlakuan sebanyak 80 ekor dengan konsentrasi 0 ppm; 259,51 ppm; 291,94 ppm; dan 324,38 ppm. Pada akhir minggu ke empat diambil sampel insang dan dilakukan analisis gambaran struktur mikroanatomi insang secara diskriptif. Hasil analisis menunjukkan kerusakan edema 0%-25%, fusi lamela antara 1%-75%, hiperplasia 0%-50%, epithelial lifting 0%-50%, dan nekrosis 0%-50%. Dari hasil penelitian dapat disimpulkan bahwa nilai LC50-96 jam pada uji toksisitas ikan Nila Larasati sebesar 324,38 ppm dan mulai terjadi perubahan struktur mikroanatomi insang pada konsentrasi 259,51 ppm. The objective of thje study was to determine the value of LC50-96 hours in a toxicity test of Nila Larasati fish exposed to lead acetate; to determine the changes in the microanatomy structure and to determine the concentration of lead acetate which causes microanatomy structure changes in the gills of Nila Larasati fish. The samples were 120 fish to find out the 50% fish mortality within 96 hours, and 80 fish in treatment test with the lead acetate concentrations of 0 ppm; 259.51 ppm; 291.94 ppm; and 324.38 ppm. At the end of the 4th week of the research, the gill samples were taken and the analysis of the microanatomy structure was carried out descriptively. The result of the analysis showed that there were 0%-25% edema, 1%-75% lamella fusion, 0%-50% hyperplasia, 0%-50% epithelial lifting and 0%-50% necrosis. It was concluded that the value of LC50-96 hours in Nila Larasati fish toxicity test was 324.38 ppm; and microanatomy changes started to occur in the lead acetate concentration of 259.51 ppm.

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