Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase

Edward King; Sarah Maxel; Yulai Zhang; Karissa C. Kenney; Youtian Cui; Emma Luu; Justin B. Siegel; Gregory A. Weiss; Ray Luo; Han Li

doi:10.1038/s41467-022-35021-x

Nature Communications (Nov 2022)

Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase

Edward King,
Sarah Maxel,
Yulai Zhang,
Karissa C. Kenney,
Youtian Cui,
Emma Luu,
Justin B. Siegel,
Gregory A. Weiss,
Ray Luo,
Han Li

Affiliations

Edward King: Department of Molecular Biology and Biochemistry, University of California Irvine
Sarah Maxel: Department Chemical and Biomolecular Engineering University of California Irvine
Yulai Zhang: Department Chemical and Biomolecular Engineering University of California Irvine
Karissa C. Kenney: Department of Chemistry, University of California Irvine
Youtian Cui: Genome Center, University of California Davis
Emma Luu: Genome Center, University of California Davis
Justin B. Siegel: Genome Center, University of California Davis
Gregory A. Weiss: Department of Molecular Biology and Biochemistry, University of California Irvine
Ray Luo: Department of Molecular Biology and Biochemistry, University of California Irvine
Han Li: Department Chemical and Biomolecular Engineering University of California Irvine

DOI: https://doi.org/10.1038/s41467-022-35021-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 14

Abstract

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Engineering enzymes to accept noncanonical cofactor biomimetics is difficult. Here, the authors establish a self-sufficient growth selection method and demonstrate its application in engineering the Lactobacillus pentosus NADH oxidase to efficiently recycle reduced nicotinamide mononucleotide (NMNH).

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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