Visual barcodes for clonal-multiplexing of live microscopy-based assays

Tom Kaufman; Erez Nitzan; Nir Firestein; Miriam Bracha Ginzberg; Seshu Iyengar; Nish Patel; Rotem Ben-Hamo; Ziv Porat; Jaryd Hunter; Andreas Hilfinger; Varda Rotter; Ran Kafri; Ravid Straussman

doi:10.1038/s41467-022-30008-0

Nature Communications (May 2022)

Visual barcodes for clonal-multiplexing of live microscopy-based assays

Tom Kaufman,
Erez Nitzan,
Nir Firestein,
Miriam Bracha Ginzberg,
Seshu Iyengar,
Nish Patel,
Rotem Ben-Hamo,
Ziv Porat,
Jaryd Hunter,
Andreas Hilfinger,
Varda Rotter,
Ran Kafri,
Ravid Straussman

Affiliations

Tom Kaufman: Department of Molecular Cell Biology, Weizmann Institute of Science
Erez Nitzan: Department of Molecular Cell Biology, Weizmann Institute of Science
Nir Firestein: Department of Molecular Cell Biology, Weizmann Institute of Science
Miriam Bracha Ginzberg: Programme in Cell Biology, The Hospital for Sick Children
Seshu Iyengar: Department of Chemical and Physical Sciences, University of Toronto
Nish Patel: Programme in Cell Biology, The Hospital for Sick Children
Rotem Ben-Hamo: Department of Molecular Cell Biology, Weizmann Institute of Science
Ziv Porat: Flow Cytometry Unit, Life Sciences Core Facilities, Weizmann Institute of Science
Jaryd Hunter: Programme in Cell Biology, The Hospital for Sick Children
Andreas Hilfinger: Department of Chemical and Physical Sciences, University of Toronto
Varda Rotter: Department of Molecular Cell Biology, Weizmann Institute of Science
Ran Kafri: Programme in Cell Biology, The Hospital for Sick Children
Ravid Straussman: Department of Molecular Cell Biology, Weizmann Institute of Science

DOI: https://doi.org/10.1038/s41467-022-30008-0
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 17

Abstract

Read online

Multiplex analyses of samples allow understanding complex processes in cancer initiation, progression and therapy response. Here, the authors present a fluorescence imaging-based visual barcode for livecell clonal-multiplexing which allows identifying signalling pathways clusters in response to different chemotherapy compounds.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal