The Magnaporthe oryzae effector Avr-PikD suppresses rice immunity by inhibiting an LSD1-like transcriptional activator
Jiayuan Guo,
Yiling Wu,
Jianqiang Huang,
Kaihui Yu,
Meilian Chen,
Yijuan Han,
Zhenhui Zhong,
Guodong Lu,
Yonghe Hong,
Zonghua Wang,
Xiaofeng Chen
Affiliations
Jiayuan Guo
Fujian Universities Engineering Research Center of Marine Biology and Drugs, Fuzhou Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou 350108, Fujian, China; Ministerial and Provincial Joint Innovation Center for Safety Production of Cross-Strait Crops, Minjiang University, Fuzhou 350108, Fujian, China; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
Yiling Wu
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
Jianqiang Huang
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
Kaihui Yu
Ministerial and Provincial Joint Innovation Center for Safety Production of Cross-Strait Crops, Minjiang University, Fuzhou 350108, Fujian, China; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
Meilian Chen
Fujian Universities Engineering Research Center of Marine Biology and Drugs, Fuzhou Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou 350108, Fujian, China; Ministerial and Provincial Joint Innovation Center for Safety Production of Cross-Strait Crops, Minjiang University, Fuzhou 350108, Fujian, China
Yijuan Han
Fujian Universities Engineering Research Center of Marine Biology and Drugs, Fuzhou Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou 350108, Fujian, China; Ministerial and Provincial Joint Innovation Center for Safety Production of Cross-Strait Crops, Minjiang University, Fuzhou 350108, Fujian, China
Zhenhui Zhong
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
Guodong Lu
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
Yonghe Hong
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350018, Fujian, China; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China; Corresponding authors.
Zonghua Wang
Fujian Universities Engineering Research Center of Marine Biology and Drugs, Fuzhou Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou 350108, Fujian, China; Ministerial and Provincial Joint Innovation Center for Safety Production of Cross-Strait Crops, Minjiang University, Fuzhou 350108, Fujian, China; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China; Corresponding authors.
Xiaofeng Chen
Fujian Universities Engineering Research Center of Marine Biology and Drugs, Fuzhou Institute of Oceanography, College of Geography and Oceanography, Minjiang University, Fuzhou 350108, Fujian, China; Ministerial and Provincial Joint Innovation Center for Safety Production of Cross-Strait Crops, Minjiang University, Fuzhou 350108, Fujian, China; Corresponding authors.
Avirulence effectors (Avrs), encoded by plant pathogens, can be recognized by plants harboring the corresponding resistance proteins, thereby initiating effector-triggered immunity (ETI). In susceptible plants, however, Avrs can function as effectors, facilitating infection via effector-triggered susceptibility (ETS). Mechanisms of Avr-mediated ETS remain largely unexplored. Here we report that the Magnaporthe oryzae effector Avr-PikD enters rice cells via the canonical cytoplasmic secretion pathway and suppresses rice basal defense. Avr-PikD interacts with an LSD1-like transcriptional activator AKIP30 of rice, and AKIP30 is also a positive regulator of rice immunity, whereas Avr-PikD impedes its nuclear localization and suppresses its transcriptional activity. In summary, M. oryzae delivers Avr-PikD into rice cells to facilitate ETS by inhibiting AKIP30-mediated transcriptional regulation of immune response against M. oryzae.
