Spectroscopic and In Vitro Investigations of Boron(III) Complex with <i>Meso</i>-4-Methoxycarbonylpropylsubstituted Dipyrromethene for Fluorescence Bioimaging Applications
Galina Guseva,
Elena Antina,
Mikhail Berezin,
Svetlana Lisovskaya,
Roman Pavelyev,
Airat Kayumov,
Olga Lodochnikova,
Daut Islamov,
Konstantin Usachev,
Sergei Boichuk,
Liliya Nikitina
Affiliations
Galina Guseva
G.A. Krestov Institute of Solution Chemistry of Russian Academy of Sciences, 1 Akademicheskaya st., 153045 Ivanovo, Russia
Elena Antina
G.A. Krestov Institute of Solution Chemistry of Russian Academy of Sciences, 1 Akademicheskaya st., 153045 Ivanovo, Russia
Mikhail Berezin
G.A. Krestov Institute of Solution Chemistry of Russian Academy of Sciences, 1 Akademicheskaya st., 153045 Ivanovo, Russia
Svetlana Lisovskaya
Kazan State Medical University, 49 Butlerova st., 420012 Kazan, Russia
Roman Pavelyev
Kazan Federal University, 18 Kremlyovskaya st., 420008 Kazan, Russia
Airat Kayumov
Kazan Federal University, 18 Kremlyovskaya st., 420008 Kazan, Russia
Olga Lodochnikova
Kazan Federal University, 18 Kremlyovskaya st., 420008 Kazan, Russia
Daut Islamov
Kazan Federal University, 18 Kremlyovskaya st., 420008 Kazan, Russia
Konstantin Usachev
Kazan Federal University, 18 Kremlyovskaya st., 420008 Kazan, Russia
Sergei Boichuk
Kazan State Medical University, 49 Butlerova st., 420012 Kazan, Russia
Liliya Nikitina
Kazan State Medical University, 49 Butlerova st., 420012 Kazan, Russia
This study focuses on the behavior of a new fluorescent marker for labeling individual biomolecules and staining cell organelles developed on a meso-substituted BODIPY platform. Boron(III) complex with meso-4-methoxycarbonylpropylsubstituted 3,3’,5,5’-tetramethyl-2,2′-dipyrromethene has been synthesized and identified via visible, UV-, NMR- and MS-spectra X-ray. The behavior of fluorophore in solutions has been studied with various experimental techniques. It has been found that luminophore exhibits a high quantum yield (almost ~100–75%) in the blue-green region (513–520 nm) and has high photostability. In addition, biological analysis indicates that the fluorophore exhibits a tendency to effectively penetrate into cell membranes. On the other hand, the proposed BODIPY can be used to study the significant differences among a large number of pathogens of mycotic infections, as well as to visualize structural changes in the plasma membrane, which is necessary for the clearance of mammalian cells undergoing apoptotic cell death.
