Journal of Agricultural Science and Sustainable Production (Jan 2015)
Grouping of Barley Genotypes Using Molecular and Morphological Markers, and Dry Matter Remobilization to Grain under Water Deficit
Abstract
Water availability at critical times of growth in crops is the most important limiting factor for economic performance in arid and semi-arid regions. Water stress tolerance in a plant genotype depends on some physiological and morphological traits including affecting components in yield stability and dry matter remobilization under drought conditions. In this study, 26 lines along to 14 varieties of winter barley were evaluated for dry matter remobilization (amount, efficiency and contribution of remobilization) and some morphological traits. Mean comparisons between genotypes showed Sahra cultivar and naked barley Chamico were the highest and lines of Dari-friz88-A-3, Dari-friz88-A-8, Dari-Dayton friz88-A-6 and Dayton were the lowest in terms of traits related to remobilization of stem and peduncle to grain during grain filling. The highest yield of seed belonged to line of F-A3-3 in the all levels of stress. This line also had high seed weight, number of grains per spike, and amount, efficiency and contribution of dry matter remobilization of stem and peduncle to grain. Genotyping was also applied using markers of AFLP, ISSR and RAPD. The genotypes were classified to four categories with range of similarity coefficients from 0.33 to 0.958. The lowest and highest genetic distance obtained among lines of F-ERB-84-11 and F-A3-3 and lines of Dari-friz-A-8 and naked barley Alanda, respectively. Results showed that AFLP markers had the most efficiency to determine polymorphism and genetic diversity among the genotypes.
