Investigation of the Properties of Immobilized Horseradish Peroxidase on Magnetic Particles

Abstract

Magnetic particles coated with copolymer of acrylamide and acrylonitrile have been prepared. Those particles were used as a matrix for a covalent binding of peroxidase. The periodic-oxidized enzyme was bound to the matrix by immobilization procedure at 4°C for 18 hours. The immobilized enzyme showed relative activity of 86%. The following results were obtained for pH and optimum temperature of the immobilized enzyme - 7.0 and 30°C, respectively. The analysis of the kinetic parameters of the immobilized enzyme showed values of Vmax - 0.0517 M.10-6/sec and Km - 2.3x10-4 M.

Keywords

• horseradish peroxidase
• magnetic particle
• covalent immobilization