Nature Communications (Aug 2019)
A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules
- Mindong Liang,
- Zilong Li,
- Weishan Wang,
- Jiakun Liu,
- Leshi Liu,
- Guoliang Zhu,
- Loganathan Karthik,
- Man Wang,
- Ke-Feng Wang,
- Zhong Wang,
- Jing Yu,
- Yuting Shuai,
- Jiaming Yu,
- Lu Zhang,
- Zhiheng Yang,
- Chuan Li,
- Qian Zhang,
- Tong Shi,
- Liming Zhou,
- Feng Xie,
- Huanqin Dai,
- Xueting Liu,
- Jingyu Zhang,
- Guang Liu,
- Ying Zhuo,
- Buchang Zhang,
- Chenli Liu,
- Shanshan Li,
- Xuekui Xia,
- Yaojun Tong,
- Yanwen Liu,
- Gil Alterovitz,
- Gao-Yi Tan,
- Li-Xin Zhang
Affiliations
- Mindong Liang
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Zilong Li
- State Key Laboratory of Microbial Resources and CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Weishan Wang
- State Key Laboratory of Microbial Resources and CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Jiakun Liu
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Leshi Liu
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Guoliang Zhu
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Loganathan Karthik
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Man Wang
- School of Agriculture and Biology, Shanghai Jiao Tong University
- Ke-Feng Wang
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Zhong Wang
- Department of Urology, Sixth People’s Hospital South Campus Affiliated to Shanghai Jiao Tong University
- Jing Yu
- Department of Clinical Laboratory, Sixth People’s Hospital South Campus Affiliated to Shanghai Jiao Tong University
- Yuting Shuai
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Jiaming Yu
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Lu Zhang
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Zhiheng Yang
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Chuan Li
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Qian Zhang
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Tong Shi
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Liming Zhou
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Feng Xie
- State Key Laboratory of Microbial Resources and CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Huanqin Dai
- State Key Laboratory of Microbial Resources and CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences
- Xueting Liu
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Jingyu Zhang
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Guang Liu
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Ying Zhuo
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Buchang Zhang
- Institute of Physical Science and Information Technology, Anhui University
- Chenli Liu
- Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences
- Shanshan Li
- State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences
- Xuekui Xia
- Key Biosensor Laboratory of Shandong Province, Biology Institute, Qilu University of Technology (Shandong Academy of Sciences)
- Yaojun Tong
- The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark
- Yanwen Liu
- inBiome Sciences
- Gil Alterovitz
- Harvard Medical School Countway Library
- Gao-Yi Tan
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- Li-Xin Zhang
- State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST)
- DOI
- https://doi.org/10.1038/s41467-019-11648-1
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 9
Abstract
Bacterial allosteric transcription factors can sense and respond to a variety of small molecules. Here the authors present CaT-SMelor which uses Cas12a and allosteric transcription factors to detect small molecules in the nanomolar range.
