Molecular and genetic analyses revealed the phytotoxicity of perfluorobutane sulfonate

Abstract

Perfluorobutane sulfonate (PFBS) has oily and hydrophobic characteristics similar to those of perfluorooctane sulfonic acid (PFOS), which is an environmental organic pollutant and has gradually become the main substitute for PFOS in industry. Several studies have revealed the potential toxicity of PFBS in animals. PFBS can be taken up and accumulate in plants; however, whether and how PFBS affects plant growth remain largely unclear. A low concentration of PFBS did not affect plant growth, indicating that it had higher environmental safety than other perfluorinated compounds; however, a high concentration of PFBS (>1 mM) markedly inhibited primary root growth in Arabidopsis thaliana. Subsequently, we investigated the molecular mechanisms underlying plant growth mediated by high concentrations of PFBS. First, a genome-wide transcriptomic analysis revealed that PFBS altered the expression of genes associated with phytohormone signaling pathways. Combining physio-biochemical and genetic analyses, we next demonstrated that PFBS reduced the contents of indole-3-acetic acid (IAA) and abscisic acid (ABA), and disrupted the two signaling pathways in plants, finally inhibiting root growth. Moreover, a high concentration of PFBS also inhibited photosynthesis by comprehensively repressing the expression of genes related to the Calvin cycle and the photosynthetic apparatus. Such an understanding is helpful for elucidating the phytotoxicity of PFBS and provides a new strategy for toxicology research on organic pollutants in plants.

Keywords

• Perfluorobutane sulfonate
• Environmental risk
• Phytohormone signaling pathways
• Primary root growth
• Photosynthesis