International Journal of Reproductive BioMedicine (Dec 2022)

Evaluation of the FAS and FASL Gene changes in women with premature ovarian failure: A case-control study

  • Zhima Akhavansales,
  • Alimohammad Mosadeghmehrjardi,
  • Hamid Reza Ashrafzadeh,
  • Shadnaz Fakhteh Yavari,
  • Mohammad Taher Tahoori,
  • Morteza Bitaraf Sani,
  • Mahnaz Mohammadi,
  • Fateme Montazeri,
  • Nasrin Ghasemi

DOI
https://doi.org/10.18502/ijrm.v20i12.12561
Journal volume & issue
Vol. 20, no. 12
pp. 1007 – 1012

Abstract

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Abstract Background: Premature ovarian failure (POF), is menopause occurring before the age of 40, affecting 1-3% of women worldwide. The risk of POF increases with altered immunological parameters such as FAS and FASL genes, which play a fundamental role in embryogenesis and cellular homeostasis. Objective: The study aimed to investigate the potential role of FAS and FASL genes in POF pathogenesis. Materials and Methods: In this case-control study, the polymorphisms of FAS-670A/G and FASLIVS2nt_124A/G apoptotic genes were analyzed in 51 Iranian women suffering from POF, and 61 healthy controls. Isolation of DNA was done using the salting-out method, and genotypic analysis was performed for all the subjects using the polymerase chain reaction-restriction fragment length polymorphism method. Results: Our results revealed that homozygous FAS-670A/A and G/G, and heterozygous FAS-670A/G are not significantly different between cases and controls (p = 0.99). Also, in different genotyping models of FASIVS2nt_124, polymorphisms were not related to POF risk (p = 0.23). Conclusion: There is no statistical association between these polymorphisms and POF risk in women referred to genetic counseling clinics.

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