THE SIDEROPHORE PRODUCTION BY PSEUDOMONAS BACTERIA

Abstract

Aim. The study of the siderophore synthesis by Pseudomonas genus bacteria. Methods. The siderophore production ability of P. aeruginosa (ONU 300, ONU 301, ONU 302), P. fluorescens ONU 303 and P. aureofaciens (ONU 304, ONU 305, ONU 306) strains was studied by using of CAS-agar. The intensity of growth and pyoverdin biochelator production of the studied cultures in liquid King medium variant was determined, respectively, at the wavelengths of 660 nm and 400 nm with using of chrome-azurols. Results. According to the intensity of siderophore accumulation by the representatives of different strains the same species distinguished from each other. The most active siderophore production was observed among P. aeruginosa and P. fluorescens cultures. The iron ions (III) that were additionally added resulted the changes both the cell quantity and siderophore synthesis intensity. Adding iron to the medium in which Pseudomonas cultured, was resulting in increased cell number in suspension. However, concentration of siderophores, which are synthesized by these microorganisms, in the presence of 30 μg/ml of iron (III) decreased by 2–7 fold, and at 1000 μg/ml – synthesis of tested compounds by most Pseudomonas strains practically completely ceased. Conclusions. Presence of iron ions (III) in nutrient medium at concentration 30 and 1000 μg/ml slowed down the accumulation of siderophores by bacteria of Pseudomonas genus.

Keywords

• сидерофори
• pseudomonas spp.
• піовердини, fe3