Cancer Management and Research (Nov 2019)

AMPH-1 As A Critical Tumor Suppressor That Inhibits Osteosarcoma Progression

  • Zhang H,
  • Liu Y,
  • Xu K,
  • Mao K,
  • Han W,
  • Xu F,
  • Wan W,
  • Sun Y

Journal volume & issue
Vol. Volume 11
pp. 9913 – 9919

Abstract

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Haiyun Zhang,1,* Yujie Liu,2,* Kehan Xu,2,* Kai Mao,1 Weidong Han,1 Feifan Xu,1 Wei Wan,2 Yajun Sun3 1Department of Laboratory Medicine, The Sixth People’s Hospital of Nantong, Jiangsu 226000, People’s Republic of China; 2Department of Orthopedic Oncology, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, People’s Republic of China; 3Department of Laboratory Medicine, The Fourth People’s Hospital of Nantong, Jiangsu 226000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yajun Sun; Wei WanDepartment of Laboratory Medicine, The Fourth People’s Hospital of Nantong, Chenggang Road, Jiangsu 226000, People’s Republic of ChinaEmail [email protected]; [email protected]: Amphiphysin 1 (AMPH-1) is involved in endocytosis, and its expression is upregulated in osteosarcoma compared with osteofibrous dysplasia.Methods: We investigated the role of AMPH-1 in osteosarcoma cells via both gain-of-function and loss-of-function experiments.Results: Knockdown of AMPH-1 in osteosarcoma cells promoted cell cycle progression and cell proliferation and attenuated apoptosis. Notably, silencing AMPH-1 increased osteosarcoma progression in a mouse tumor model. The results obtained upon AMPH-1 knockdown and AMPH-1 overexpression indicates that AMPH-1 is involved in regulating MEK/ERK signaling.Conclusion: These data suggest that AMPH-1 plays an important role in osteosarcoma and may represent a novel therapeutic target for osteosarcoma treatment.Keywords: AMPH-1, osteosarcoma, ERK signaling pathway

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