Site-specific photolabile roadblocks for the study of transcription elongation in biologically complex systems

Jean-François Nadon; Vitaly Epshtein; Etienne Cameron; Mikhail R. Samatov; Andrey S. Vasenko; Evgeny Nudler; Daniel A. Lafontaine

doi:10.1038/s42003-022-03382-0

Communications Biology (May 2022)

Site-specific photolabile roadblocks for the study of transcription elongation in biologically complex systems

Jean-François Nadon,
Vitaly Epshtein,
Etienne Cameron,
Mikhail R. Samatov,
Andrey S. Vasenko,
Evgeny Nudler,
Daniel A. Lafontaine

Affiliations

Jean-François Nadon: Department of Biology, Faculty of Science, RNA Group, Université de Sherbrooke
Vitaly Epshtein: Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine
Etienne Cameron: Department of Chemical Engineering, Polytechnique Montreal
Mikhail R. Samatov: HSE University
Andrey S. Vasenko: HSE University
Evgeny Nudler: Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine
Daniel A. Lafontaine: Department of Biology, Faculty of Science, RNA Group, Université de Sherbrooke

DOI: https://doi.org/10.1038/s42003-022-03382-0
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 12

Abstract

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Transcriptional pausing can be achieved by 6-nitropiperonyloxymethyl modification, which can halt RNAP without causing backtracking and be efficiently removed by short illumination with a moderately intense UV light.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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