A potential mechanism of miana (Coleus scutellariodes) and quercetin via NF-κB in Salmonella typhi infection
Ade Rifka Junita,
Firdaus Hamid,
Budu Budu,
Rosdiana Natzir,
Yusmina Hala,
Gemini Alam,
Rosana Agus,
Burhanuddin Bahar,
Ahmad Syukri,
Muhammad Reza Primaguna,
Ressy Dwiyanti,
Andini Febrianti,
Muhammad Sabir,
Azhar Azhar,
Mochammad Hatta
Affiliations
Ade Rifka Junita
Postgraduate School, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia; Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Firdaus Hamid
Department of Microbiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Budu Budu
Department of Ophthalmology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Rosdiana Natzir
Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Yusmina Hala
Department of Biology, Faculty of Sciences, State University of Makassar, Makassar, Indonesia
Gemini Alam
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia
Rosana Agus
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia
Burhanuddin Bahar
Department of Biostatistic, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Ahmad Syukri
Postgraduate School, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia; Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Muhammad Reza Primaguna
Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Ressy Dwiyanti
Department of Medical Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia; Department of Forensic and Medicolegal, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Andini Febrianti
Department of Forensic and Medicolegal, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Muhammad Sabir
Department of Medical Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Azhar Azhar
Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Mochammad Hatta
Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia; Corresponding author. Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, 90245, Indonesia.
Purpose: To prove the effect of Miana (M), Quercetin (Q), and the combination as an anti-inflammatory agent and Cefixime (C) as an antibiotic in Balb/c mice infected with Salmonella enterica serovar Typhi (S. Typhi) and related to the dynamics of NF-κB mRNA expression and NF-κB protein levels. Methods: A cohort study on male Balb/c mice with subjects consisted of 8 groups with 5 each group by administration of M, Q, M + Q, M + C, Q + C, M + Q + C, C only and sterile distilled water group as negative control. The statistical significance of the difference group was defined as P values less than 0.05. Results: Decreased mRNA expression of NF-κB, NF-κB protein levels, and bacterial load after administration of M + C, Q + C, or M + Q + C showed significant differences when compared to the negative control. The decline in NF-κB was stronger when M + Q + C was given compared to M, Q, M + Q, or C only. Conclusion: The effects of NF-κB suppression appear to be the same between the administration of M, Q and the M + Q when C added. However, the suppression of NF-κB was not significant without adding C.
