Infection and Drug Resistance (Dec 2019)

Diagnostic Accuracy of Interleukin-27 in Bronchoalveolar Lavage Fluids for Pulmonary Tuberculosis

  • Lin S,
  • Wang Y,
  • Li Y,
  • Xiao D,
  • Guo J,
  • Ma W,
  • An W,
  • Liu H,
  • Shi Y,
  • Zhang L,
  • Cui J,
  • Guan W

Journal volume & issue
Vol. Volume 12
pp. 3755 – 3763

Abstract

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Shan Lin,1,2,* Yan Wang,1,* Yuhong Li,1 Di Xiao,1 Jin Guo,1 Weixiu Ma,1 Wenjing An,1 Hongqian Liu,1 Yingqing Shi,1 Lei Zhang,1 Jingxia Cui,1 Wei Guan1,3,* 1Department of Respiratory Medicine, Qinghai University Affiliated Hospital, Xining 810001, People’s Republic of China; 2Department of Medical Intensive Care Unit, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, GuangDong 510080, People’s Republic of China; 3Department of Respiratory and Critical Care Medicine, Baoan Central Hospital of Shenzhen/The Fifth Affiliated Hospital of Shenzhen University, Shenzhen, GuangDong 518102, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wei GuanDepartment of Respiratory and Critical Care Medicine, Baoan Central Hospital of Shenzhen/The Fifth Affiliated Hospital of Shenzhen University, 60 Xixiang Leyuan Street, Shenzhen, GuangDong 518102, People’s Republic of ChinaEmail [email protected] CuiDepartment of Respiratory Medicine, Qinghai University Affiliated Hospital, Xining 810001, People’s Republic of ChinaEmail [email protected]: The World Health Organization states that China had 0.9 million cases of tuberculosis in 2017, accounting for 9% of cases globally. Despite a decrease in the incidence and mortality of tuberculosis in China over time, development in choosing the appropriate prevention and control of TB is required.Purpose: The aim of this study was to evaluate the diagnostic significance of interleukin-27 in bronchoalveolar lavage fluids for pulmonary tuberculosis.Materials and methods: Eventually, 107 bronchoalveolar lavage fluids from patients were included in this study. The concentrations of interleukin-27 and adenosine deaminase were determined in bronchoalveolar lavage fluids using enzyme-linked immunosorbent assay.Results: It was found that the concentrations of interleukin-27 in bronchoalveolar lavage fluids of sputum-positive pulmonary tuberculosis group were significantly higher than those in sputum-negative pulmonary tuberculosis, lung cancer, and previous pulmonary tuberculosis groups, respectively (all P<0.001). Interleukin-27 levels in bronchoalveolar lavage fluids could be used for diagnostic purpose for pulmonary tuberculosis, with the cutoff value of 7.867 pg/mL; interleukin-27 had a sensitivity of 68.8% and specificity of 100% for the differential diagnosis of pulmonary tuberculosis (sputum-negative and sputum-positive PTB) from lung cancer. And with the cutoff value of 6.012 pg/mL, IL-27 had sensitivity and specificity of both 100% for the differential diagnosis of PTB from previous PTB. The risk of pulmonary tuberculosis was positively associated with the concentrations of interleukin-27 and adenosine deaminase in bronchoalveolar lavage fluids.Conclusion: Interleukin-27 in bronchoalveolar lavage fluids is a sensitive and specific biomarker for the differential diagnosis of pulmonary tuberculosis from lung cancer and previous pulmonary tuberculosis.Keywords: pulmonary tuberculosis, interleukin-27, adenosine deaminase, bronchoalveolar lavage fluid

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