The Levels of Polycyclic Aromatic Hydrocarbons and Their Derivatives in Plasma and Their Effect on Mitochondrial DNA Methylation in the Oilfield Workers
Yaning Jia,
Weixia Li,
Yanlin Li,
Lei Zhao,
Chenguang Li,
Lei Wang,
Junkai Fang,
Shanjun Song,
Yaqin Ji,
Tao Fang,
Jing Zhang,
Liqiong Guo,
Penghui Li
Affiliations
Yaning Jia
Institute of Disaster and Emergency Medicine, Tianjin University, Tianjin 300072, China
Weixia Li
Institute of Disaster and Emergency Medicine, Tianjin University, Tianjin 300072, China
Yanlin Li
Tianjin Boshengyuan Environmental Technology Center, Tianjin 300381, China
Lei Zhao
Institute of Disaster and Emergency Medicine, Tianjin University, Tianjin 300072, China
Chenguang Li
School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China
Lei Wang
Hebei Research Center for Geoanalysis, Baoding 071000, China
Junkai Fang
Tianjin Institute of Medical & Pharmaceutical Sciences, Tianjin 300070, China
Shanjun Song
National Institute of Metrology, Beijing 100029, China
Yaqin Ji
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China
Tao Fang
Institute of Disaster and Emergency Medicine, Tianjin University, Tianjin 300072, China
Jing Zhang
School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China
Liqiong Guo
Institute of Disaster and Emergency Medicine, Tianjin University, Tianjin 300072, China
Penghui Li
School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China
This study focuses on the components and levels of polycyclic aromatic hydrocarbons (PAHs) and their derivatives (MPAHs and OPAHs) in plasma samples from 19 oil workers, pre- and post-workshift, and their exposure–response relationship with mitochondrial DNA (mtDNA) methylation. PAH, MPAH, OPAH, and platelet mtDNA methylation levels were determined using a gas chromatograph mass spectrometer (GC-MS) and a pyrosequencing protocol, respectively. The total plasma concentrations of PAHs in mean value were, respectively, 31.4 ng/mL and 48.6 ng/mL in pre- and post-workshift, and Phe was the most abundant (13.3 ng/mL in pre-workshift and 22.1 ng/mL in post-workshift, mean value). The mean values of total concentrations of MPAHs and OPAHs in the pre-workshift were 2.7 ng/mL and 7.2 ng/mL, while in the post-workshift, they were 4.5 ng/mL and 8.7 ng/mL, respectively. The differences in the mean MT-COX1, MT-COX2, and MT-COX3 methylation levels between pre- and post-workshift were 2.36%, 5.34%, and 0.56%. Significant (p MT-COX1 (β = 0.831, SD = 0.105, p MT-COX3 (β = 0.115, SD = 0.042, p β = 0.036, SD = 0.015, p < 0.05, respectively). The results indicated that exposure to PAHs was an independent factor influencing mtDNA methylation.
