不同饱和度脂肪酸豆甾醇酯的体外生物可给率In vitro bioaccessibility of fatty acid stigmasterol esters with different saturation

Abstract

为了探究脂肪酸饱和度对其植物甾醇酯生物可给率的影响，采用体外模拟消化静态模型，系统研究了油酸豆甾醇酯、亚油酸豆甾醇酯和亚麻酸豆甾醇酯的体外模拟消化规律。结果表明：体外模拟消化结束后（120 min),亚油酸豆甾醇酯游离脂肪酸（FFA）释放率最高，为78.51%，其次是亚麻酸豆甾醇酯，为74.21%，油酸豆甾醇酯FFA释放率最低，为69.15%；3种豆甾醇酯的水解速率由大到小为亚麻酸豆甾醇酯>亚油酸豆甾醇酯>油酸豆甾醇酯；3种豆甾醇酯的水解度由大到小为亚油酸豆甾醇酯>亚麻酸豆甾醇酯>油酸豆甾醇酯；油酸豆甾醇酯、亚油酸豆甾醇酯和亚麻酸豆甾醇酯生物可给率分别为14.01%、11.11%和10.19%，总体差异较小。综上，豆甾醇酯中结合态的脂肪酸（相同碳链长度）饱和度影响其水解速率，但对豆甾醇酯生物可给率影响较小。 In order to investigate the effect of fatty acid saturation on the bioaccessibility of phytosterol ester, a static model of in vitro digestion was used to study the digestion characteristics of stigmasterol oleate, stigmasterol linoleate and stigmasterol linolenate. The results showed that after the simulated digestion in vitro (120 min), the free fatty acid (FFA) release rate of stigmasterol linoleate was the highest with 78.51%, followed by stigmasterol linolenate with 74.21%, and that of stigmasterol oleate was the lowest with 69.15%. The hydrolysis rates of the three stigmasterol esters decreased in the order: stigmasterol linolenate > stigmasterol linoleate> stigmasterol oleate. The degree of hydrolysis of stigmasterol esters decreased in the order: stigmasterol linoleate > stigmasterol linolenate > stigmasterol oleate. The bioaccessibilities of stigmasterol oleate, stigmasterol linoleate and stigmasterol linolenate were 14.01%, 11.11% and 10.19% respectively, and the overall difference was small. In conclusion, the degree of saturation of bound fatty acids (same carbon chain length) in stigmasterol esters affects the hydrolysis rate, but has little effect on the bioaccessibility of stigmasterol esters.

Keywords

• 植物甾醇酯；脂肪酸饱和度；体外模拟消化；生物可给率
• phytosterol ester; fatty acid saturation; in vitro digestion; bioaccessibility