Creation of a patient-like model of esophageal cancer in immunodeficient mice

Abstract

Introduction. To study the antitumor effect of substances in vivo, it is necessary to create a model that reflects the main characteristics of a human disease. Patient-like xenografts (Patient Derived Xenograft, PDX) on immunodeficient mice can meet this requirement. Patient-like tumor models are considered the most progressive because of their ability to maintain their initial properties, characterized by diversity at the histological and molecular level, after implantation. The aim of the study was to create a PDX model of human esophageal cancer in the cervical esophagus of immunodeficient mice.Material and Methods. To create a PDX-model of human esophageal cancer, 11 male Balb/c Nude immunodeficient mice weighing 19-22 g were used. A tumor fragment was taken from a patient with a diagnosis of ulcerative cancer - a moderately differentiated squamous cell carcinoma without keratinization.Results. As a result of implantation of a donor tumor fragment, complete engraftment of the material was observed in 6 (54.5 %) out of 11 animals, which is consistent with published data. according with the results of morphological studies, a patient-like xenograft is a squamous cell carcinoma, which histologically corresponds to the patient's tumor.

Keywords

• esophageal cancer
• patient-like model
• immunodeficient mice
• squamous cell carcinoma