Guangdong nongye kexue (Oct 2023)
Construction of Factory Breeding Technology System for Tissue Cultured Seedlings of Mussaenda hybird
Abstract
【Objective】An efficient factory breeding technology system for tissue cultured seedlings of Mussaenda hybird was established to provide technical support for the large-scale production of the variety.【Method】The stem tips and stem segments of the mother plant of M. hybird were used as explants to study the effects of different disinfection types and treatments, basic medium types and hormone ratios and light quality on the reproductive efficiency of their seedlings. And the effects of different seasons on the survival rate and growth of transplanted seedlings were explored.【Result】Explants of M. hybird were easy to die when exposed to alcohol, and sterile water washing could reduce the toxicity of alcohol to explants; 10% H2O2 treatment for 10 min and 0.1% HgCl2 treatment for 8 min could obtain a large number of sterile materials. Explants had high bud induction rate and strong buds when cultured on 3/2MS + 6-BA 3.0 mg/L + NAA 0.2 mg/L. According to the results of the effects of proliferation methods under different formulations on factory production efficiency of tissue cultured seedlings, propagation of M. hybird was more efficient on 3/2MS + 6-BA 0.5 mg/L + NAA 0.1 mg/L + peptone 0.1 g/L by stems; the rooting rate of tissue cultured seedlings was 100% on 3/2 MS + NAA 0.04-0.05 mg/L + IBA 0.01 mg/L, the roots were strong, and the plants grew well. There were significant differences in the effects of different seasons on the domestication and transplanting of tissue cultured seedlings of M. hybird. It was found that significant differences in the growth and development and chlorophyll content of tissue culture seedlings with different light qualities. Fluorescent lamp treatment was suitable for proliferation and propagation, and LED with 80% red light +20% blue light was suitable for strong seedlings cultivation at rooting stage.【Conclusion】Effective disinfection method of explants, and suitable medium formula and light quality conditions for the induction and proliferation of buds were obtained, which provided a theoretical basis for the factory breeding of Mussaenda hybird.
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