Zhongguo shuxue zazhi (Apr 2023)
Therapeutic effects of IVIG on oxaliplatin-induced spleen and intestinal injuries in mice
Abstract
Objective To investigate the effects of intravenous immunoglobulin (IVIG) on oxaliplatin-induced chemotherapy injury in mice. Methods Fifty-one wild-type male BALB/c mice aged 6-8 weeks were randomly divided into three groups: 1) the control group; 2) the chemotherapy injury group: the mice were injected with oxaliplatin; 3) the treatment group: mice were treated with IVIG after oxaliplatin injection.The effect of IVIG on oxaliplatin-induced chemotherapy injury was evaluated by comparing the differences in the cell composition of peripheral blood, splenic index, pathological features of spleen and colon and colon inflammatory cytokines, which were detected by peripheral blood cell counts, anatomy, hematoxylin-eosin staining, periodic acid-schiff staining and Luminex, respectively. Results Compared with the control group, the counts of white blood cell (109/L, 2.45±0.51 vs 0.75±0.27, P<0.000 1) and platelet (109/L, 1 249.00±100.04 vs 347.55±110.33, P<0.000 1) and the proportion of lymphocytes (75.37%±7.30% vs 62.57%±12.09%, P<0.001) in peripheral blood descend significantly in the chemotherapy injury group. The splenic index remarkably declined (P<0.000 1) and the number of goblet cells was also decreased (P<0.05) in chemotherapy-injured mice. Furthermore, the proinflammatory factors TNF-α and IFN-γ increased (P<0.01) and the anti-inflammatory factors IL-10 and IL-13 decreased (P<0.05) in chemotherapy-injured mice. Compared with the chemotherapy injury group, IVIG treatment greatly increased the numbers of white blood cell (109/L, 0.75±0.27 vs 1.11±0.30, P<0.05) and platelet (109/L, 347.55±110.33 vs 698.49±182.86, P<0.000 1) in peripheral blood of mice, and raised the proportion of lymphocytes (62.57%±12.09% vs 71.40%±9.08%, P<0.05). In addition, IVIG not only increased the spleen index of mice(P<0.000 1), the number of goblet cells and the levels of IL-10 and IL-13 in the colon of mice(P<0.05), but also decreased the colonic proinflammatory factor TNF-α levels(P<0.05). Conclusion IVIG repaired the damages of hematopoietic system, spleen and intestinal tract in mice injured by oxaliplatin, suggesting that IVIG will be effective for treating oxaliplatin-induced injuries in tumor patients.
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