PLoS ONE (Jan 2016)

AtGRP3 Is Implicated in Root Size and Aluminum Response Pathways in Arabidopsis.

  • Amanda Mangeon,
  • Renan Pardal,
  • Adriana Dias Menezes-Salgueiro,
  • Guilherme Leitão Duarte,
  • Ricardo de Seixas,
  • Fernanda P Cruz,
  • Vanessa Cardeal,
  • Claudia Magioli,
  • Felipe Klein Ricachenevsky,
  • Rogério Margis,
  • Gilberto Sachetto-Martins

DOI
https://doi.org/10.1371/journal.pone.0150583
Journal volume & issue
Vol. 11, no. 3
p. e0150583

Abstract

Read online

AtGRP3 is a glycine-rich protein (GRP) from Arabidopsis thaliana shown to interact with the receptor-like kinase AtWAK1 in yeast, in vitro and in planta. In this work, phenotypic analyses using transgenic plants were performed in order to better characterize this GRP. Plants of two independent knockout alleles of AtGRP3 develop longer roots suggesting its involvement in root size determination. Confocal microscopy analysis showed an abnormal cell division and elongation in grp3-1 knockout mutants. Moreover, we also show that grp3-1 exhibits an enhanced Aluminum (Al) tolerance, a feature also described in AtWAK1 overexpressing plants. Together, these results implicate AtGRP3 function root size determination during development and in Al stress.