Investigation of the Anti-Inflammatory Activity of Hydroxyzine Hihydrochloride in Mammalian Macrophages on the PI3K and P38 Pathway

Abstract

Objective: Depression is a common mental disorder that affects patients' health-related quality of life. Hydroxyzine hydrochloride (HHCL), which is among the antihistamines, is generally used in allergic treatments. Also, HHCL, has been routinely used in the treatment of anxiety, mental distress and stress in recent years. Although there are many studies on hydroxyzine chloride, there is not enough information in the literature about the effects of this drug on the immune system. Materials and Methods: Previous studies suggest that some antidepressants are anti-inflammatory and anti-analgesic. Therefore, the immunomodulatory activities of one of these drugs, Hydroxyzine hydrochloride, on mammalian macrophages were analyzed in flow cytometry to determine the possible intracellular mechanisms of action of the phosphorylated (active) p38 MAPK and PI3K proteins. Results: It has been found that Hydroxyzine hydrochloride applied to macrophage cells causes a significant decrease in the levels of p38 MAPK and PI3K proteins. Conclusion: According to these data, Hydroxyzine hydrochloride (HHCL), had different effects on macrophages. While HHCL triggers a mild inflammatory response in non-activated macrophages; it had anti-inflammatory effects on already activated macrophages. Intracellular mechanisms of action of HHCL were partially via the p38 MAPK and PI3K pathways.

Keywords

• anti-inflammatory activity
• hydroxyzine hydrochloride
• macrophages
• ; inflammation
• depression
• antiinflamatuar aktivite
• hidroksizin hidroklorür
• makrofajlar
• enflamasyon
• depresyon