Sensor Based on a Poly[2-(Dimethylamino)ethyl Methacrylate-<i>Co</i>-Styrene], Gold Nanoparticles, and Methylene Blue-Modified Glassy Carbon Electrode for Melamine Detection

Abstract

Melamine has been used as a non-protein nitrogenous additive in food products to artificially increase the apparent “false” protein content. Melamine is known as a dangerous and poisonous substance for human health and it causes diverse diseases. An electrochemical sensor for melamine detection has been developed by modification of a glassy carbon electrode using copolymer poly[DMAEMA-co-styrene], gold nanoparticles, and methylene blue. The characterization of the modified electrode was conducted using several analysis techniques including cyclic voltammetry (CV), differential pulse voltammetry (DPV), chronoamperometry (CA), and electrochemical impedance spectroscopy (EIS). The electrochemical detection of melamine was performed by impedance spectroscopy. Obtained results revealed that the developed sensor has a large detection range from 5.0 × 10−13 to 3.8 × 10−8 M with a low detection limit of 1.8 × 10−12 M (at S/N = 3). Various interfering species such as phenol, hydroquinone, and bisphenol A have been used and their behavior on modified electrode has been studied.

Keywords

• electrochemical sensor
• gold nanoparticles
• melamine
• methylene blue
• glassy carbon electrode
• modified electrode