Rice Ragged Stunt Virus Propagation and Infection on Rice Plants
Chao Zhang,
Chaonan Shi,
Dong Chen,
Jianguo Wu
Affiliations
Chao Zhang
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Vector-borne Virus Research Center, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, China
Chaonan Shi
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Vector-borne Virus Research Center, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, China
Dong Chen
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Vector-borne Virus Research Center, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, China
Jianguo Wu
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Vector-borne Virus Research Center, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, China
Virus inoculation is a basic experimental procedure to evaluate the resistance of a rice variety or a transgenic material upon virus infection. We recently demonstrated that Rice Ragged Stunt Virus (RRSV), an oryzavirus that is transmitted by brown planthopper (BPH), can suppress jasmonic acid-mediated antiviral defense through the induction of microRNA319 and facilitate virus infection in rice. To verify this, we performed virus inoculation experiments on wild-type rice plants and miR319-TCP21-associated transgenic rice plants through a modified group inoculation method. Here, we presented the detailed procedure of RRSV propagation and infection process on rice plants.
