Frontiers in Immunology (Dec 2022)
Two distinct resident macrophage populations coexist in the ovary
- Nianyu Li,
- Nianyu Li,
- Nianyu Li,
- Nianyu Li,
- Nianyu Li,
- Nianyu Li,
- Zhuqing Li,
- Zhuqing Li,
- Zhuqing Li,
- Zhuqing Li,
- Zhuqing Li,
- Zhuqing Li,
- Fang Fang,
- Fang Fang,
- Fang Fang,
- Fang Fang,
- Fang Fang,
- Fang Fang,
- Chendi Zhu,
- Chendi Zhu,
- Chendi Zhu,
- Chendi Zhu,
- Chendi Zhu,
- Chendi Zhu,
- Wenzhe Zhang,
- Wenzhe Zhang,
- Wenzhe Zhang,
- Wenzhe Zhang,
- Wenzhe Zhang,
- Wenzhe Zhang,
- Yueshuang Lu,
- Yueshuang Lu,
- Yueshuang Lu,
- Yueshuang Lu,
- Yueshuang Lu,
- Yueshuang Lu,
- Rongrong Zhang,
- Rongrong Zhang,
- Rongrong Zhang,
- Rongrong Zhang,
- Rongrong Zhang,
- Rongrong Zhang,
- Pinxin Si,
- Pinxin Si,
- Pinxin Si,
- Pinxin Si,
- Pinxin Si,
- Pinxin Si,
- Yuehong Bian,
- Yuehong Bian,
- Yuehong Bian,
- Yuehong Bian,
- Yuehong Bian,
- Yuehong Bian,
- Yingying Qin,
- Yingying Qin,
- Yingying Qin,
- Yingying Qin,
- Yingying Qin,
- Yingying Qin,
- Xue Jiao,
- Xue Jiao,
- Xue Jiao,
- Xue Jiao,
- Xue Jiao,
- Xue Jiao,
- Xue Jiao
Affiliations
- Nianyu Li
- Center for Reproductive Medicine, Shandong University, Jinan, Shandong, China
- Nianyu Li
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong, China
- Nianyu Li
- Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, China
- Nianyu Li
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong, China
- Nianyu Li
- Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong, China
- Nianyu Li
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong, China
- Zhuqing Li
- Center for Reproductive Medicine, Shandong University, Jinan, Shandong, China
- Zhuqing Li
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong, China
- Zhuqing Li
- Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, China
- Zhuqing Li
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong, China
- Zhuqing Li
- Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong, China
- Zhuqing Li
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong, China
- Fang Fang
- Center for Reproductive Medicine, Shandong University, Jinan, Shandong, China
- Fang Fang
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong, China
- Fang Fang
- Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, China
- Fang Fang
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong, China
- Fang Fang
- Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong, China
- Fang Fang
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong, China
- Chendi Zhu
- Center for Reproductive Medicine, Shandong University, Jinan, Shandong, China
- Chendi Zhu
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong, China
- Chendi Zhu
- Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, China
- Chendi Zhu
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong, China
- Chendi Zhu
- Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong, China
- Chendi Zhu
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong, China
- Wenzhe Zhang
- Center for Reproductive Medicine, Shandong University, Jinan, Shandong, China
- Wenzhe Zhang
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong, China
- Wenzhe Zhang
- Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, China
- Wenzhe Zhang
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong, China
- Wenzhe Zhang
- Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong, China
- Wenzhe Zhang
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong, China
- Yueshuang Lu
- Center for Reproductive Medicine, Shandong University, Jinan, Shandong, China
- Yueshuang Lu
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong, China
- Yueshuang Lu
- Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, China
- Yueshuang Lu
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong, China
- Yueshuang Lu
- Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong, China
- Yueshuang Lu
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong, China
- Rongrong Zhang
- Center for Reproductive Medicine, Shandong University, Jinan, Shandong, China
- Rongrong Zhang
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong, China
- Rongrong Zhang
- Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, China
- Rongrong Zhang
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong, China
- Rongrong Zhang
- Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong, China
- Rongrong Zhang
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong, China
- Pinxin Si
- Center for Reproductive Medicine, Shandong University, Jinan, Shandong, China
- Pinxin Si
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong, China
- Pinxin Si
- Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, China
- Pinxin Si
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong, China
- Pinxin Si
- Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong, China
- Pinxin Si
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong, China
- Yuehong Bian
- Center for Reproductive Medicine, Shandong University, Jinan, Shandong, China
- Yuehong Bian
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong, China
- Yuehong Bian
- Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, China
- Yuehong Bian
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong, China
- Yuehong Bian
- Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong, China
- Yuehong Bian
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong, China
- Yingying Qin
- Center for Reproductive Medicine, Shandong University, Jinan, Shandong, China
- Yingying Qin
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong, China
- Yingying Qin
- Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, China
- Yingying Qin
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong, China
- Yingying Qin
- Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong, China
- Yingying Qin
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong, China
- Xue Jiao
- Center for Reproductive Medicine, Shandong University, Jinan, Shandong, China
- Xue Jiao
- Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong, China
- Xue Jiao
- Shandong Key Laboratory of Reproductive Medicine, Jinan, Shandong, China
- Xue Jiao
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong, China
- Xue Jiao
- Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong, China
- Xue Jiao
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong, China
- Xue Jiao
- Suzhou Institute of Shandong University, Suzhou, Jiangsu, China
- DOI
- https://doi.org/10.3389/fimmu.2022.1007711
- Journal volume & issue
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Vol. 13
Abstract
IntroductionTissue-resident macrophages (TRMs) are highly heterogeneous and have a complex and important role in tissue support, homeostasis, and function. The heterogeneity, maintenance, and function of TRMs, as one of the major immune cells in the ovary, are not well understood.MethodsApplication of flow cytometry, Parabiosis, Fate mapping, Macrophage depletion, etc.ResultsHere, we described two distinct macrophage subsets, F4/80hiCD11bint and F4/80intCD11bhi, with different phenotypic characteristics in the ovary of mice. The F4/80hiCD11bint population contained a distinct CD206+ subgroup and highly expressed CD81, while the F4/80intCD11bhi subset showed higher expression of CCR2 and TLR2. Notably, Ly6c+ macrophages were present almost exclusively in the F4/80intCD11bhi subpopulation. Combining in vivo fate mapping and parabiotic mouse models, we characterized the longevity and replenishment of the two macrophage populations. We found that both the F4/80hiCD11bint and F4/80intCD11bhi subsets were ovary-resident. Importantly, the F4/80hiCD11bint macrophages acted as a self-maintaining and long-lived population with a modest monocyte contribution at a steady state, and the F4/80intCD11bhi subpopulation had a relatively short lifespan with a greater contribution from monocytes. After macrophage ablation, disturbance of estradiol secretion and ovarian hemorrhage due to damaged vascular integrity was observed in mice.DiscussionOur data provide critical insights into ovarian macrophage heterogeneity and highlight the strategic role of TRMs in ovarian homeostasis and physiology.
Keywords
